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Micellar Enzymology for Thermal, pH, and Solvent Stability.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1504, pp. 19-23. - Publication Year :
- 2017
-
Abstract
- This chapter describes methods for enzyme stabilization using micellar solutions. Micellar solutions have been shown to increase the thermal stability, as well as the pH and solvent tolerance of enzymes. This field is traditionally referred to as micellar enzymology. This chapter details the use of ionic and nonionic micelles for the stabilization of polyphenol oxidase, lipase, and catalase, although this method could be used with any enzymatic system or enzyme cascade system.
- Subjects :
- Agaricales chemistry
Animals
Cattle
Chromobacterium chemistry
Enzyme Stability
Hydrogen-Ion Concentration
Kinetics
Lipids chemistry
Liver enzymology
Solvents chemistry
Surface-Active Agents chemistry
Temperature
Agaricales enzymology
Catalase chemistry
Catechol Oxidase chemistry
Chromobacterium enzymology
Lipase chemistry
Micelles
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1504
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 27770411
- Full Text :
- https://doi.org/10.1007/978-1-4939-6499-4_3