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Secretome profiling of highly virulent Mycobacterium bovis 04-303 strain reveals higher abundance of virulence-associated proteins.
- Source :
-
Microbial pathogenesis [Microb Pathog] 2016 Nov; Vol. 100, pp. 305-311. Date of Electronic Publication: 2016 Oct 18. - Publication Year :
- 2016
-
Abstract
- Mycobacterium bovis is the causative agent of tuberculosis in farms, wildlife and causes sporadic disease in humans. Despite the high similitude in genome sequence between M. bovis strains, some strains like the wild boar 04-303 isolate show a highly virulent phenotype in animal models. Comparative studies will contribute to link protein expression with the virulence phenotype. In vitro, the 04-303 strain was more phagocytized by J774A.1 macrophages in comparison with 444 strain (a cow isolate with the same genotype) and BCG. The secretome of these strains showed a significant proportion of shared proteins (368 spots). Among the proteins only visualized in the secretome of the 04-303 strain, we identify the nine most abundant proteins by LC-MS/MS. The most relevant were EsxA and EsxB proteins, which are encoded in the RD1 region, deleted in BCG strains. These proteins are the major virulence factor of M. tuberculosis. The other proteins identified belong to functional categories of virulence, detoxification, and adaptation; lipid metabolism; and cell wall and cell processes. The relatively high proportion of proteins involved in the cell wall and cell process is consistent with the previously described variation among M. bovis genomes.<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Chromatography, Liquid
Macrophages immunology
Macrophages microbiology
Mycobacterium bovis immunology
Mycobacterium bovis isolation & purification
Mycobacterium tuberculosis
Phagocytosis
Sus scrofa
Tandem Mass Spectrometry
Bacterial Proteins analysis
Bacterial Proteins metabolism
Mycobacterium bovis chemistry
Mycobacterium bovis metabolism
Proteome analysis
Virulence Factors analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1096-1208
- Volume :
- 100
- Database :
- MEDLINE
- Journal :
- Microbial pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 27769937
- Full Text :
- https://doi.org/10.1016/j.micpath.2016.10.014