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Oral Administration of Linoleic Acid Induces New Vessel Formation and Improves Skin Wound Healing in Diabetic Rats.
- Source :
-
PloS one [PLoS One] 2016 Oct 20; Vol. 11 (10), pp. e0165115. Date of Electronic Publication: 2016 Oct 20 (Print Publication: 2016). - Publication Year :
- 2016
-
Abstract
- Introduction: Impaired wound healing has been widely reported in diabetes. Linoleic acid (LA) accelerates the skin wound healing process in non-diabetic rats. However, LA has not been tested in diabetic animals.<br />Objectives: We investigated whether oral administration of pure LA improves wound healing in streptozotocin-induced diabetic rats.<br />Methods: Dorsal wounds were induced in streptozotocin-induced type-1 diabetic rats treated or not with LA (0.22 g/kg b.w.) for 10 days. Wound closure was daily assessed for two weeks. Wound tissues were collected at specific time-points and used to measure fatty acid composition, and contents of cytokines, growth factors and eicosanoids. Histological and qPCR analyses were employed to examine the dynamics of cell migration during the healing process.<br />Results: LA reduced the wound area 14 days after wound induction. LA also increased the concentrations of cytokine-induced neutrophil chemotaxis (CINC-2αβ), tumor necrosis factor-α (TNF-α) and leukotriene B4 (LTB4), and reduced the expression of macrophage chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1 (MIP-1). These results together with the histological analysis, which showed accumulation of leukocytes in the wound early in the healing process, indicate that LA brought forward the inflammatory phase and improved wound healing in diabetic rats. Angiogenesis was induced by LA through elevation in tissue content of key mediators of this process: vascular-endothelial growth factor (VEGF) and angiopoietin-2 (ANGPT-2).<br />Conclusions: Oral administration of LA hastened wound closure in diabetic rats by improving the inflammatory phase and angiogenesis.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Administration, Oral
Angiopoietin-2 metabolism
Animals
Cell Movement drug effects
Cytokines metabolism
Gene Expression Regulation drug effects
Linoleic Acid pharmacology
Rats
Streptozocin
Vascular Endothelial Growth Factor A metabolism
Diabetes Mellitus, Experimental complications
Linoleic Acid administration & dosage
Neovascularization, Physiologic drug effects
Wound Healing drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 11
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 27764229
- Full Text :
- https://doi.org/10.1371/journal.pone.0165115