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Impact of Collagen/Heparin Multilayers for Regulating Bone Cellular Functions.
- Source :
-
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2016 Nov 09; Vol. 8 (44), pp. 29923-29932. Date of Electronic Publication: 2016 Oct 28. - Publication Year :
- 2016
-
Abstract
- Bone cell interaction with extracellular matrix (ECM) microenvironment is of critical importance when engineering surface interfaces for bone regeneration. In this work layer-by-layer films of type I collagen (coll), the major constituent of bone ECM, and heparin (hep), a glycosaminoglycan, were assembled on poly(l-lactic acid) (PLLA) substrates to evaluate the impact of the biomacromolecular coating on cell activity. The surface modification of PLLA demonstrated that the hep/coll multilayer is stable after 10 bilayers (confirmed by contact angle, infrared spectroscopy, and morphological analysis). This simple approach provided novel information on the effect of heparin on type I collagen hierarchical organization and subsequent cell response of osteoblast-like (MC3T3-E1) and human bone marrow-derived mesenchymal stem cells (hMSCs). Interestingly, the number of deposited heparin layers (1 or 10) appeared to play an important role in the self-assembly of collagen into fibrils, stabilizing the fibrous collagen layer, and potentially impacting hMSCs activity.
Details
- Language :
- English
- ISSN :
- 1944-8252
- Volume :
- 8
- Issue :
- 44
- Database :
- MEDLINE
- Journal :
- ACS applied materials & interfaces
- Publication Type :
- Academic Journal
- Accession number :
- 27762547
- Full Text :
- https://doi.org/10.1021/acsami.6b09241