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Predictive role BLVRA mRNA expression in hepatocellular cancer.
- Source :
-
Annals of hepatology [Ann Hepatol] 2016 Nov-Dec 2016; Vol. 15 (6), pp. 881-887. - Publication Year :
- 2016
-
Abstract
- Introduction and aim. Hepatocellular carcinoma (HCC) is the most common primary malignant liver tumor. It is primarily caused by hepatic cirrhosis or chronic viral hepatitis. Hepatic carcinogenesis is associated with increased oxidative stress. Thus, the aim of our study was to assess expression of the genes involved in the homeostasis of oxidative stress in patients with HCC.<br />Material and Methods: The study was performed on 32 patients with primary HCC (verified by liver histology in 29 patients) and 27 control subjects (in 11 subjects, liver histology was available either with no or minimal changes in the liver tissue). Gene expressions of heme oxygenase 1 (HMOX1), biliverdin reductase A/B (BLVRA/B), NADPH oxidase 2 (NOX2) and p22phox were analyzed in the liver and peripheral blood leukocytes (PBL) in the subjects.<br />Results: Compared to controls, almost a 3 times higher mRNA level of BLVRA was detected in livers of HCC patients (p = 0.002); while those of BLVRB as well as HMOX1 were unchanged (p > 0.05). In accord with these results in the liver tissue, BLVRA mRNA levels in PBL were also significantly increased in HCC patients (p = 0.012). mRNA levels of NOX2 and p22phox in the liver tissue, although higher in HCC patients, did not differ significantly compared to control subjects (p > 0.05). Nevertheless, NOX2 mRNA level in PBL was significantly higher in HCC patients (p = 0.003).<br />Conclusions: BLVRA mRNA levels in the liver as well as in PBL are significantly higher in HCC patients most likely as a feedback mechanism to control increased oxidative stress associated with HCC progression.
- Subjects :
- Aged
Biomarkers, Tumor blood
Carcinoma, Hepatocellular blood
Carcinoma, Hepatocellular enzymology
Carcinoma, Hepatocellular pathology
Case-Control Studies
Disease Progression
Female
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic
Heme Oxygenase-1 genetics
Humans
Liver Neoplasms blood
Liver Neoplasms enzymology
Liver Neoplasms pathology
Male
Membrane Glycoproteins genetics
Middle Aged
NADPH Oxidase 2
NADPH Oxidases genetics
Oxidative Stress genetics
Oxidoreductases Acting on CH-CH Group Donors blood
Signal Transduction
Up-Regulation
Biomarkers, Tumor genetics
Carcinoma, Hepatocellular genetics
Liver Neoplasms genetics
Oxidoreductases Acting on CH-CH Group Donors genetics
RNA, Messenger genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1665-2681
- Volume :
- 15
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Annals of hepatology
- Publication Type :
- Academic Journal
- Accession number :
- 27740521
- Full Text :
- https://doi.org/10.5604/16652681.1222104