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Microencapsulated Cells for Cancer Therapy.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1479, pp. 261-272. - Publication Year :
- 2017
-
Abstract
- The microencapsulation of different types of cells that are able to produce therapeutic factors is being investigated for the treatment of several human diseases. Most efforts are focused on chronic and degenerative diseases as this strategy could become an alternative to some commonly used parenteral treatments that need to be repeatedly administered. But, this approach has also been investigated in the field of oncology with the aim of providing immunomodulatory antibodies that are able to enhance the patient's inherent immune response against the tumor. These kind of treatments would provide the patient with the therapeutic drug produced in situ, de novo, and in a sustained way, making the therapy more comfortable.Although different devices are nowadays available to produce cell-enclosing alginate-microcapsules, here, we describe the most important steps and advices in order to fabricate alginate-poly-L-lysine-alginate microcapsules containing hybridoma cells for cancer management using an electrostatic bead generator, and how to evaluate the viability of those cells over the time.
- Subjects :
- Animals
Cell Count
Cell Line
Cell Survival
Cell- and Tissue-Based Therapy methods
Cells, Immobilized metabolism
Cells, Immobilized transplantation
Drug Compounding instrumentation
Drug Compounding methods
Drug Delivery Systems
Equipment Design
Humans
Hybridomas metabolism
Hybridomas transplantation
Polylysine chemistry
Static Electricity
Alginates chemistry
Capsules chemistry
Cells, Immobilized cytology
Hybridomas cytology
Neoplasms therapy
Polylysine analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1479
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 27738943
- Full Text :
- https://doi.org/10.1007/978-1-4939-6364-5_21