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Luminescent properties of 4-aminobenzo-15-crown-5 after preferential binding of ferric ions in aqueous solutions.
- Source :
-
Luminescence : the journal of biological and chemical luminescence [Luminescence] 2017 Jun; Vol. 32 (4), pp. 549-554. Date of Electronic Publication: 2016 Oct 12. - Publication Year :
- 2017
-
Abstract
- We report a combined approach that introduces the use of 4-aminobenzo-15-crown-5 (4AB15C5) for the detection of ferric(III) ions by colorimetric, ultraviolet (UV)-visible light absorption, fluorescence, and live-cell imaging techniques along with density functional theory (DFT) calculations. We have found that 4AB15C5 is sensitive and selective for binding ferric(III) ions in aqueous solutions. DFT calculations using the polarizable continuum model have been used to explain the strong binding of the ferric ion by 4AB15C5 in aqueous solutions. The detection limit in the fluorescence quenching measurements was found to be as low as 50 μM for the ferric ion with a determined Stern-Volmer constant of 1.52 × 10 <superscript>4</superscript>  M <superscript>-1</superscript> . Fluorescence intensity did not change for other ions tested, Fe <superscript>2</superscript> <superscript>+</superscript> , Co <superscript>2</superscript> <superscript>+</superscript> , Mn <superscript>2</superscript> <superscript>+</superscript> , Mg <superscript>2</superscript> <superscript>+</superscript> , Zn <superscript>2</superscript> <superscript>+</superscript> , Ca <superscript>2</superscript> <superscript>+</superscript> , NH <subscript>4</subscript> <superscript>+</superscript> , Na <superscript>+</superscript> , and K <superscript>+</superscript> ions. Live-cell fluorescence imaging was also used to check the intracellular variations in ferric ion levels. Our spectroscopic data indicated that 4AB15C5 can bind ferric ions selectively in aqueous solutions.<br /> (Copyright © 2016 John Wiley & Sons, Ltd.)
- Subjects :
- Aniline Compounds chemistry
Cations metabolism
Crown Compounds chemistry
Fluorescence
HeLa Cells
Humans
Iron chemistry
Limit of Detection
Luminescent Agents metabolism
Luminescent Measurements
Microscopy, Fluorescence
Molecular Imaging methods
Sensitivity and Specificity
Solutions
Spectrophotometry, Ultraviolet
Water chemistry
Aniline Compounds metabolism
Crown Compounds metabolism
Iron analysis
Iron metabolism
Luminescent Agents chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1522-7243
- Volume :
- 32
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Luminescence : the journal of biological and chemical luminescence
- Publication Type :
- Academic Journal
- Accession number :
- 27730739
- Full Text :
- https://doi.org/10.1002/bio.3220