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Bergenin attenuates renal injury by reversing mitochondrial dysfunction in ethylene glycol induced hyperoxaluric rat model.
- Source :
-
European journal of pharmacology [Eur J Pharmacol] 2016 Nov 15; Vol. 791, pp. 611-621. Date of Electronic Publication: 2016 Oct 05. - Publication Year :
- 2016
-
Abstract
- Bergenin, isolated from Bergenia ligulata is a potent antioxidant and antilithiatic agent. Present work was designed to establish the biochemical role of bergenin on mitochondrial dysfunction in the ethylene glycol induced hyperoxaluric rat model. Bergenin was administrated at a dose of 10mg/kg body wt i.p. from 14th day of establishing the 28 days hyperoxaluria rat model. α-Tocopherol was given as positive control at a dose of 100mg/kg body wt i.p. Mitochondrial dysfunction was studied by evaluating the activities of respiratory chain complexes, mitochondrial membrane potential and reactive oxygen species. Histopathological analysis of the kidney tissue was done after Pizzolato staining. Also, expression of monocyte chemoattractant protein -1(MCP-1) and kidney injury marker protein (KIM-1) were studied and the levels of IL-1β were evaluated in kidney tissue homogenate. Mitochondrial dysfunction during stone crystallization was evident by decreased activities of electron transport chain complexes I, II and IV and augmented mitochondrial oxidative stress in hyperoxaluric rats. Bergenin treatment significantly (P<0.05) restored the activities of these complexes. Moreover, it curtailed the lipid peroxidation and up regulated antioxidant levels, ameliorating the state of mitochondrial dysfunction. The protective role of bergenin was also reinforced by reducing IL-1β production and expression of KIM-1 and MCP-1 in the renal tissue. The findings of the present study provide evidence that bergenin exerted protective effects in hyperoxaluria through mitochondrial protection that involves attenuation of oxidative stress. Hence, it presented itself as an effective remedy in combating urolithiasis.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Antioxidants pharmacology
Antioxidants therapeutic use
Benzopyrans therapeutic use
Biomarkers metabolism
Chemokine CCL2 genetics
Chemokine CCL2 metabolism
Creatinine metabolism
Cytoprotection drug effects
Disease Models, Animal
Gene Expression Regulation drug effects
Hyperoxaluria drug therapy
Hyperoxaluria metabolism
Interleukin-1beta metabolism
Kidney metabolism
Kidney pathology
L-Lactate Dehydrogenase metabolism
Male
Membrane Potential, Mitochondrial drug effects
Mitochondria pathology
Oxidation-Reduction drug effects
Oxidative Stress drug effects
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species metabolism
Benzopyrans pharmacology
Ethylene Glycol pharmacology
Hyperoxaluria chemically induced
Hyperoxaluria pathology
Kidney drug effects
Kidney injuries
Mitochondria drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0712
- Volume :
- 791
- Database :
- MEDLINE
- Journal :
- European journal of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 27717728
- Full Text :
- https://doi.org/10.1016/j.ejphar.2016.10.002