Back to Search
Start Over
Nanoscopic compartmentalization of membrane protein motion at the axon initial segment.
- Source :
-
The Journal of cell biology [J Cell Biol] 2016 Oct 10; Vol. 215 (1), pp. 37-46. Date of Electronic Publication: 2016 Oct 03. - Publication Year :
- 2016
-
Abstract
- The axon initial segment (AIS) is enriched in specific adaptor, cytoskeletal, and transmembrane molecules. During AIS establishment, a membrane diffusion barrier is formed between the axonal and somatodendritic domains. Recently, an axonal periodic pattern of actin, spectrin, and ankyrin forming 190-nm-spaced, ring-like structures has been discovered. However, whether this structure is related to the diffusion barrier function is unclear. Here, we performed single-particle tracking time-course experiments on hippocampal neurons during AIS development. We analyzed the mobility of lipid-anchored molecules by high-speed single-particle tracking and correlated positions of membrane molecules with the nanoscopic organization of the AIS cytoskeleton. We observe a strong reduction in mobility early in AIS development. Membrane protein motion in the AIS plasma membrane is confined to a repetitive pattern of ∼190-nm-spaced segments along the AIS axis as early as day in vitro 4, and this pattern alternates with actin rings. Mathematical modeling shows that diffusion barriers between the segments significantly reduce lateral diffusion along the axon.<br /> (© 2016 Albrecht et al.)
- Subjects :
- Animals
Cell Tracking
Cells, Cultured
Diffusion
Glycosylphosphatidylinositols metabolism
Green Fluorescent Proteins metabolism
Hippocampus cytology
Neurons cytology
Neurons metabolism
Quantum Dots metabolism
Rats, Sprague-Dawley
Spectrin metabolism
Axon Initial Segment metabolism
Cell Compartmentation
Membrane Proteins metabolism
Nanoparticles chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1540-8140
- Volume :
- 215
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 27697928
- Full Text :
- https://doi.org/10.1083/jcb.201603108