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Molecular and cellular effects of vitamin B12 forms on human trophoblast cells in presence of excessive folate.
- Source :
-
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2016 Dec; Vol. 84, pp. 526-534. Date of Electronic Publication: 2016 Sep 28. - Publication Year :
- 2016
-
Abstract
- Folic acid (FA) and iron are essential supplements during pregnancy. Similarly effects of vitamin B12 (B12) inadequacy and high folate and low B12 status, on pregnancy outcome are available. However there are no mandatory recommendations for B12. There are many forms of B12 viz. Cyanocobalamin (Cbl), Methylcobalamin (MeCbl), Adenosylcobalamin (AdCbl), and Hydroxycobalamin (HCbl) though there is limited consensus on which form has better efficacy. In the present study we have determined effect of various forms of B12 in the presence of two FA concentrations namely normal physiological (20ng/mL; NPFA) and supra-physiological (2000ng/mL; SPFA) concentration to mimic real time situation where FA is in excess due to supplementation. We assessed trophoblastic proliferation, viability, TNFα and EGFr mRNA expression, homocysteine, β-hCG and MDA levels. Trophoblastic viability was significantly suppressed at SPFA concentration and was restored by B12 treatment with Cbl, AdCbl and combination of MeCbl+AdCbl. The mRNA expressions of TNFα were up-regulated, while EGFr were down-regulated at SPFA concentrations, as validated by RT-PCR. Treatment with MeCbl+AdCbl significantly decreased homocysteine and MDA levels at SPFA concentrations. High levels of FA alone had a detrimental effect on placental health and functions as reflected by decreased viability, EGFr expression and increased TNFα expression, homocysteine and MDA levels. Combination of B12 active forms i.e. MeCbl+AdCbl was found to be most effective in neutralising excess folate effect in-vitro.<br /> (Copyright © 2016 Elsevier Masson SAS. All rights reserved.)
- Subjects :
- Cell Line, Tumor
Cell Proliferation drug effects
Cell Survival drug effects
Chorionic Gonadotropin, beta Subunit, Human metabolism
Cytoprotection
Dose-Response Relationship, Drug
ErbB Receptors genetics
ErbB Receptors metabolism
Female
Folic Acid toxicity
Homocysteine metabolism
Humans
Hydroxocobalamin pharmacology
Malondialdehyde metabolism
Pregnancy
Trophoblasts metabolism
Trophoblasts pathology
Tumor Necrosis Factor-alpha genetics
Tumor Necrosis Factor-alpha metabolism
Vitamin B 12 pharmacology
Cobamides pharmacology
Dietary Supplements toxicity
Folic Acid pharmacology
Protective Agents pharmacology
Trophoblasts drug effects
Vitamin B 12 analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1950-6007
- Volume :
- 84
- Database :
- MEDLINE
- Journal :
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
- Publication Type :
- Academic Journal
- Accession number :
- 27693961
- Full Text :
- https://doi.org/10.1016/j.biopha.2016.09.071