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Fibroblast growth factor 2 alters the oxytocin receptor in a developmental model of anxiety-like behavior in male rat pups.
- Source :
-
Hormones and behavior [Horm Behav] 2016 Nov; Vol. 86, pp. 64-70. Date of Electronic Publication: 2016 Sep 28. - Publication Year :
- 2016
-
Abstract
- We aimed to determine the short-term effects of early-life stress in the form of maternal separation (MS) on anxiety-like behavior in male rat pups. In order to assess anxiety, we measured 40kHz separation-induced ultrasonic vocalizations (USV) on postnatal day (PND) 11. We further aimed to evaluate the potential involvement of two neurochemical systems known to regulate social and anxiety-like behaviors throughout life: oxytocin (OT) and fibroblast growth factor 2 (FGF2). For these purposes, we tested the effects of neonatal administration (on PND1) of an acute dose of FGF2 on USV and its potential interaction with MS. In addition, we validated the anxiolytic effects of OT and measured oxytocin receptor (OTR) gene expression, binding and epigenetic regulation via histone acetylation. Our results show that MS potentiated USV while acute administration of OT and FGF2 attenuated them. Further, we found that both FGF2 and MS increased OTR gene expression and the association of acH3K14 with the OTR promoter in the bed nucleus of the stria terminalis (BNST). Comparable changes, though not as pronounced, were also found for the central amygdala (CeA). Our findings suggest that FGF2 may exert its anxiolytic effects in male MS rats by a compensatory increase in the acetylation of the OTR promoter to overcome reduced OT levels in the BNST.<br /> (Copyright © 2016. Published by Elsevier Inc.)
- Subjects :
- Animals
Animals, Newborn
Anxiety metabolism
Anxiety physiopathology
Central Amygdaloid Nucleus drug effects
Central Amygdaloid Nucleus growth & development
Central Amygdaloid Nucleus metabolism
Epigenesis, Genetic drug effects
Female
Gene Expression Regulation, Developmental drug effects
Growth and Development genetics
Male
Oxytocin metabolism
Oxytocin pharmacology
Rats
Rats, Sprague-Dawley
Receptors, Oxytocin metabolism
Septal Nuclei growth & development
Septal Nuclei metabolism
Anxiety genetics
Behavior, Animal drug effects
Fibroblast Growth Factor 2 pharmacology
Growth and Development drug effects
Maternal Deprivation
Receptors, Oxytocin genetics
Septal Nuclei drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1095-6867
- Volume :
- 86
- Database :
- MEDLINE
- Journal :
- Hormones and behavior
- Publication Type :
- Academic Journal
- Accession number :
- 27693608
- Full Text :
- https://doi.org/10.1016/j.yhbeh.2016.09.009