Back to Search
Start Over
An endovascular model of ischemic myopathy from peripheral arterial disease.
- Source :
-
Journal of vascular surgery [J Vasc Surg] 2017 Sep; Vol. 66 (3), pp. 891-901. Date of Electronic Publication: 2016 Sep 29. - Publication Year :
- 2017
-
Abstract
- Objective: Peripheral arterial disease (PAD) is a significant age-related medical condition with limited pharmacologic options. Severe PAD, termed critical limb ischemia, can lead to amputation. Skeletal muscle is the end organ most affected by PAD, leading to ischemic myopathy and debility of the patient. Currently, there are not any therapeutics to treat ischemic myopathy, and proposed biologic agents have not been optimized owing to a lack of preclinical models of PAD. Because a large animal model of ischemic myopathy may be useful in defining the optimal dosing and delivery regimens, the objective was to create and to characterize a swine model of ischemic myopathy that mimics patients with severe PAD.<br />Methods: Yorkshire swine (N = 8) underwent acute right hindlimb ischemia by endovascular occlusion of the external iliac artery. The effect of ischemia on limb function, perfusion, and degree of ischemic myopathy was quantified by weekly gait analysis, arteriography, hindlimb blood pressures, femoral artery duplex ultrasound scans, and histologic examination. Animals were terminated at 5 (n = 5) and 6 (n = 3) weeks postoperatively. Ossabaw swine (N = 8) fed a high-fat diet were used as a model of metabolic syndrome for comparison of arteriogenic recovery and validation of ischemic myopathy.<br />Results: There was persistent ischemia in the right hindlimb, and occlusion pressures were significantly depressed compared with the untreated left hindlimb out to 6 weeks (systolic blood pressure, 31 ± 21 vs 83 ± 15 mm Hg, respectively; P = .0007). The blood pressure reduction resulted in a significant increase of ischemic myopathy in the gastrocnemius muscle in the treated limb. Gait analysis revealed a functional deficit of the right hindlimb immediately after occlusion that improved rapidly during the first 2 weeks. Peak systolic velocity values in the right common femoral artery were severely diminished throughout the entire study (P < .001), and the hemodynamic environment after occlusion was characterized by low and oscillatory wall shear stress. Finally, the internal iliac artery on the side of the ischemic limb underwent significant arteriogenic remodeling (1.8× baseline) in the Yorkshire but not in the Ossabaw swine model.<br />Conclusions: This model uses endovascular technology to produce the first durable large animal model of ischemic myopathy. Acutely (first 2 weeks), this model is associated with impaired gait but no tissue loss. Chronically (2-6 weeks), this model delivers persistent ischemia, resulting in ischemic myopathy similar to that seen in PAD patients. This model may be of use for testing novel therapeutics including biologic therapies for promoting neovascularization and arteriogenesis.<br /> (Published by Elsevier Inc.)
- Subjects :
- Angiography
Animals
Blood Flow Velocity
Constriction, Pathologic
Disease Models, Animal
Female
Femoral Artery diagnostic imaging
Femoral Artery pathology
Gait
Hindlimb
Humans
Iliac Artery diagnostic imaging
Iliac Artery pathology
Ischemia diagnostic imaging
Ischemia pathology
Ischemia physiopathology
Muscle, Skeletal pathology
Muscle, Skeletal physiopathology
Peripheral Arterial Disease diagnostic imaging
Peripheral Arterial Disease pathology
Peripheral Arterial Disease physiopathology
Regional Blood Flow
Severity of Illness Index
Stents
Sus scrofa
Time Factors
Ultrasonography, Doppler, Duplex
Vascular Remodeling
Endovascular Procedures instrumentation
Femoral Artery physiopathology
Hemodynamics
Iliac Artery physiopathology
Ischemia etiology
Muscle, Skeletal blood supply
Peripheral Arterial Disease etiology
Subjects
Details
- Language :
- English
- ISSN :
- 1097-6809
- Volume :
- 66
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of vascular surgery
- Publication Type :
- Academic Journal
- Accession number :
- 27693032
- Full Text :
- https://doi.org/10.1016/j.jvs.2016.07.127