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Integrin-linked kinase regulates cadherin switch in bladder cancer.
- Source :
-
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine [Tumour Biol] 2016 Nov; Vol. 37 (11), pp. 15185-15191. Date of Electronic Publication: 2016 Sep 28. - Publication Year :
- 2016
-
Abstract
- Cadherin switch is specific of epithelial-mesenchymal transition (EMT) and is closely related to tumor cell invasion. However, the molecular mechanism that promotes the phenotypic changes remains unclear and elusive. We found that integrin-linked kinase (ILK) is a key factor involved in cadherin switch. The expression and activity of ILK are elevated in a variety of cancers but its mechanisms are not exactly understood. In this report, we studied the role and mechanism of ILK in EMT of human bladder cancer. We showed that silencing of ILK expression by small interfering RNA (siRNA) significantly abolished the nuclear translocation or the presence of markers associated with EMT like Snail, Twist, Zeb, and beta-catenin. ILK knockdown by siRNA suppressed N-cadherin expression and increased re-expression of E-cadherin in bladder cancer cells. We suggest that ILK is a major signaling factor involved in EMT. It is essential to understand the molecular mechanism of EMT in aim to possibly use it in search for new therapeutic targets.<br />Competing Interests: Compliance with ethical standards Conflicts of interest None.
- Subjects :
- Antigens, CD genetics
Blotting, Western
Cadherins antagonists & inhibitors
Cadherins genetics
Cell Membrane metabolism
Cell Nucleus metabolism
Cytoplasm metabolism
Epithelial-Mesenchymal Transition
Humans
Protein Serine-Threonine Kinases antagonists & inhibitors
Protein Serine-Threonine Kinases genetics
RNA, Messenger genetics
RNA, Small Interfering genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Tumor Cells, Cultured
Urinary Bladder Neoplasms genetics
Antigens, CD metabolism
Cadherins metabolism
Gene Expression Regulation, Neoplastic
Protein Serine-Threonine Kinases metabolism
Urinary Bladder Neoplasms metabolism
Urinary Bladder Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0380
- Volume :
- 37
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 27683053
- Full Text :
- https://doi.org/10.1007/s13277-016-5354-x