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Sensitive inexpensive HPLC determination of four antiepileptic drugs in human plasma: application to PK studies.
- Source :
-
Bioanalysis [Bioanalysis] 2016 Nov; Vol. 8 (21), pp. 2219-2234. Date of Electronic Publication: 2016 Sep 28. - Publication Year :
- 2016
-
Abstract
- Aim: Recently, polytherapy regimen has been introduced for the treatment of epileptic patients for better seizure control with lesser side effects and better control of multiple seizure types.<br />Methodology: A simple, sensitive and highly specific reversed-phase HPLC method was developed for simultaneous determination of four antiepileptic drugs (AEDs), levetiracetam, lamotrigine, oxcarbazepine and carbamazepine, in real human plasma without interference from endogenous components of plasma.<br />Conclusion: The method was proved to be linear in the range of 0.5-50 µg/ml for all drugs. It was successfully applied for clinical PK study of the AEDs in healthy volunteers following single administration. Also, this method was applied for simultaneous determination of the studied drugs in volunteers' plasma receiving synergistic binary combinations from the four AEDs when used as add-on therapy. The good precision and selectivity of the developed method allow it to be used for routine therapeutic drug monitoring of such drugs as a useful tool in epilepsy management.
- Subjects :
- Anticonvulsants pharmacokinetics
Anticonvulsants standards
Calibration
Carbamazepine analogs & derivatives
Carbamazepine blood
Carbamazepine pharmacokinetics
Carbamazepine standards
Half-Life
Humans
Lamotrigine
Levetiracetam
Limit of Detection
Oxcarbazepine
Piracetam analogs & derivatives
Piracetam blood
Piracetam pharmacokinetics
Piracetam standards
Triazines blood
Triazines pharmacokinetics
Triazines standards
Anticonvulsants blood
Blood Chemical Analysis methods
Chromatography, High Pressure Liquid standards
Subjects
Details
- Language :
- English
- ISSN :
- 1757-6199
- Volume :
- 8
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Bioanalysis
- Publication Type :
- Academic Journal
- Accession number :
- 27678163
- Full Text :
- https://doi.org/10.4155/bio-2016-0211