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Discovery of a Mosaic-Like Biosynthetic Assembly Line with a Decarboxylative Off-Loading Mechanism through a Combination of Genome Mining and Imaging.
- Source :
-
Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2016 Oct 17; Vol. 55 (43), pp. 13611-13614. Date of Electronic Publication: 2016 Sep 26. - Publication Year :
- 2016
-
Abstract
- The biosynthetic gene cluster for the antiplasmodial natural product siphonazole was identified by using a combination of genome mining, imaging, and expression studies in the natural producer Herpetosiphon sp. B060. The siphonazole backbone is assembled from an unusual starter unit from the shikimate pathway that is extended by the action of polyketide synthases and non-ribosomal peptide synthetases with unusual domain structures, including several split modules and a large number of duplicated domains and domains predicted to be inactive. Product release proceeds through decarboxylation and dehydration independent of the thioesterase SphJ and yields the diene terminus of siphonazole. High variation in terms of codon-usage within the gene cluster, together with the dislocated domain organization, suggest a recent emergence in evolutionary terms.<br /> (© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Antimalarials chemistry
Antimalarials isolation & purification
Biological Products chemistry
Biological Products isolation & purification
Chloroflexi metabolism
Data Mining
Decarboxylation
Mass Spectrometry
Multigene Family
Oxazoles chemistry
Oxazoles isolation & purification
Antimalarials metabolism
Biological Products metabolism
Chloroflexi genetics
Oxazoles metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1521-3773
- Volume :
- 55
- Issue :
- 43
- Database :
- MEDLINE
- Journal :
- Angewandte Chemie (International ed. in English)
- Publication Type :
- Academic Journal
- Accession number :
- 27667141
- Full Text :
- https://doi.org/10.1002/anie.201606655