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A conserved glycine residue in the C-terminal region of human ATG9A is required for its transport from the endoplasmic reticulum to the Golgi apparatus.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2016 Oct 14; Vol. 479 (2), pp. 404-409. Date of Electronic Publication: 2016 Sep 20. - Publication Year :
- 2016
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Abstract
- ATG9A is the only polytopic protein of the mammalian autophagy-related protein family whose members regulate autophagosome formation during macroautophagy. At steady state, ATG9A localizes to several intracellular sites, including the Golgi apparatus, endosomes and the plasma membrane, and it redistributes towards autophagosomes upon autophagy induction. Interestingly, the transport of yeast Atg9 to the pre-autophagosomal structure depends on its self-association, which is mediated by a short amino acid motif located in the C-terminal region of the protein. Here, we investigated whether the residues that align with this motif in human ATG9A (V <superscript>515</superscript> -C <superscript>519</superscript> ) are also required for its trafficking in mammalian cells. Interestingly, our findings support that human ATG9A self-interacts as well, and that this process promotes transport of ATG9A molecules through the Golgi apparatus. Furthermore, our data reveal that the transport of ATG9A out of the ER is severely impacted after mutation of the conserved V <superscript>515</superscript> -C <superscript>519</superscript> motif. Nevertheless, the mutated ATG9A molecules could still interact with each other, indicating that the molecular mechanism of self-interaction differs in mammalian cells compared to yeast. Using sequential amino acid substitutions of glycine 516 and cysteine 519, we found that the stability of ATG9A relies on both of these residues, but that only the former is required for efficient transport of human ATG9A from the endoplasmic reticulum to the Golgi apparatus.<br /> (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Subjects :
- Alanine chemistry
Amino Acid Motifs
Autophagy-Related Proteins genetics
Cell Membrane metabolism
Cysteine chemistry
Endoplasmic Reticulum metabolism
Gene Deletion
Golgi Apparatus metabolism
HeLa Cells
Humans
Membrane Proteins genetics
Microscopy, Fluorescence
Protein Domains
Protein Transport
Vesicular Transport Proteins genetics
Autophagy-Related Proteins metabolism
Glycine chemistry
Membrane Proteins metabolism
Vesicular Transport Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 479
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 27663665
- Full Text :
- https://doi.org/10.1016/j.bbrc.2016.09.097