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Hydrogen-rich Water Exerting a Protective Effect on Ovarian Reserve Function in a Mouse Model of Immune Premature Ovarian Failure Induced by Zona Pellucida 3.
- Source :
-
Chinese medical journal [Chin Med J (Engl)] 2016 Oct 05; Vol. 129 (19), pp. 2331-7. - Publication Year :
- 2016
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Abstract
- Background: Premature ovarian failure (POF) is a disease that affects female fertility but has few effective treatments. Ovarian reserve function plays an important role in female fertility. Recent studies have reported that hydrogen can protect male fertility. Therefore, we explored the potential protective effect of hydrogen-rich water on ovarian reserve function through a mouse immune POF model.<br />Methods: To set up immune POF model, fifty female BALB/c mice were randomly divided into four groups: Control (mice consumed normal water, n = 10), hydrogen (mice consumed hydrogen-rich water, n = 10), model (mice were immunized with zona pellucida glycoprotein 3 [ZP3] and consumed normal water, n = 15), and model-hydrogen (mice were immunized with ZP3 and consumed hydrogen-rich water, n = 15) groups. After 5 weeks, mice were sacrificed. Serum anti-Müllerian hormone (AMH) levels, granulosa cell (GC) apoptotic index (AI), B-cell leukemia/lymphoma 2 (Bcl-2), and BCL2-associated X protein (Bax) expression were examined. Analyses were performed using SPSS 17.0 (SPSS Inc., Chicago, IL, USA) software.<br />Results: Immune POF model, model group exhibited markedly reduced serum AMH levels compared with those of the control group (5.41 ± 0.91 ng/ml vs. 16.23 ± 1.97 ng/ml, P = 0.033) and the hydrogen group (19.65 ± 7.82 ng/ml, P = 0.006). The model-hydrogen group displayed significantly higher AMH concentrations compared with that of the model group (15.03 ± 2.75 ng/ml vs. 5.41 ± 0.91 ng/ml, P = 0.021). The GC AI was significantly higher in the model group (21.30 ± 1.74%) than those in the control (7.06 ± 0.27%), hydrogen (5.17 ± 0.41%), and model-hydrogen groups (11.24 ± 0.58%) (all P < 0.001). The GC AI was significantly higher in the model-hydrogen group compared with that of the hydrogen group (11.24 ± 0.58% vs. 5.17 ± 0.41%, P = 0.021). Compared with those of the model group, ovarian tissue Bcl-2 levels increased (2.18 ± 0.30 vs. 3.01 ± 0.33, P = 0.045) and the Bax/Bcl-2 ratio decreased in the model-hydrogen group.<br />Conclusions: Hydrogen-rich water may improve serum AMH levels and reduce ovarian GC apoptosis in a mouse immune POF model induced by ZP3.
- Subjects :
- Animals
Anti-Mullerian Hormone blood
Apoptosis drug effects
Female
Granulosa Cells cytology
Hydrogen chemistry
Mice
Mice, Inbred BALB C
Ovarian Reserve physiology
Ovary drug effects
Ovary metabolism
Primary Ovarian Insufficiency blood
Proto-Oncogene Proteins c-bcl-2 metabolism
Water administration & dosage
Zona Pellucida drug effects
Zona Pellucida physiology
bcl-2-Associated X Protein metabolism
Hydrogen pharmacology
Ovarian Reserve drug effects
Primary Ovarian Insufficiency metabolism
Primary Ovarian Insufficiency prevention & control
Water chemistry
Water pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 2542-5641
- Volume :
- 129
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- Chinese medical journal
- Publication Type :
- Academic Journal
- Accession number :
- 27647193
- Full Text :
- https://doi.org/10.4103/0366-6999.190668