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Evaluation of antibacterial and antibiofilm mechanisms by usnic acid against methicillin-resistant Staphylococcus aureus.
- Source :
-
Future microbiology [Future Microbiol] 2016 Oct; Vol. 11, pp. 1315-1338. Date of Electronic Publication: 2016 Sep 16. - Publication Year :
- 2016
-
Abstract
- Aim: To evaluate the antibacterial and antibiofilm mechanisms of usnic acid (USN) against methicillin-resistant Staphylococcus aureus from cystic fibrosis patients.<br />Materials & Methods: The effects exerted by USN at subinhibitory concentrations on S. aureus Sa3 strain was evaluated by proteomic, real-time PCR and electron microscopy analyses.<br />Results & Conclusion: Proteomic analysis showed that USN caused damage in peptidoglycan synthesis, as confirmed by microscopy. Real-time PCR analysis showed that antibiofilm activity of USN is mainly due to impaired adhesion to the host matrix binding proteins, and decreasing lipase and thermonuclease expression. Our data show that USN exerts anti-staphylococcal effects through multitarget inhibitory effects, thus confirming the rationale for considering it 'lead compound' for the treatment of cystic fibrosis infections.
- Subjects :
- Adhesins, Bacterial drug effects
Anti-Bacterial Agents administration & dosage
Bacterial Proteins antagonists & inhibitors
Bacterial Proteins drug effects
Bacterial Proteins genetics
Bacterial Proteins metabolism
Benzofurans administration & dosage
Carrier Proteins drug effects
Cell Membrane drug effects
Cell Survival drug effects
Colony Count, Microbial
Cystic Fibrosis drug therapy
Cystic Fibrosis microbiology
DNA, Bacterial
Down-Regulation
Lipase drug effects
Methicillin-Resistant Staphylococcus aureus genetics
Methicillin-Resistant Staphylococcus aureus pathogenicity
Methicillin-Resistant Staphylococcus aureus ultrastructure
Microbial Sensitivity Tests
Microbial Viability drug effects
Microscopy, Electron, Scanning methods
Microscopy, Electron, Transmission methods
Peptidoglycan biosynthesis
Peptidoglycan drug effects
Propidium metabolism
Protein Interaction Maps
Proteomics methods
Real-Time Polymerase Chain Reaction methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods
Staphylococcal Infections microbiology
Time Factors
Virulence drug effects
Virulence genetics
Anti-Bacterial Agents pharmacology
Benzofurans pharmacology
Biofilms drug effects
Methicillin-Resistant Staphylococcus aureus drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1746-0921
- Volume :
- 11
- Database :
- MEDLINE
- Journal :
- Future microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 27633726
- Full Text :
- https://doi.org/10.2217/fmb-2016-0049