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Transferrin functionalized chitosan-PEG nanoparticles for targeted delivery of paclitaxel to cancer cells.
Transferrin functionalized chitosan-PEG nanoparticles for targeted delivery of paclitaxel to cancer cells.
- Source :
-
Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2016 Dec 01; Vol. 148, pp. 363-370. Date of Electronic Publication: 2016 Aug 31. - Publication Year :
- 2016
-
Abstract
- The present investigation was aimed to utilize the stealth property of polyethylene glycol (PEG) modified chitosan nanoparticles (NPs) and active targeting function of transferrin (Tf) by transferrin receptor-mediated endocytosis to promote drug delivery to cancer cells. Paclitaxel (PTX) loaded nanoparticles (PTX-NP) were prepared by solvent evaporation method; PEGylation was carried out by coupling amine group present on the surface of NPs with hydroxyl group present on the PEG (NP-PEG). Tf conjugation was carried out by coupling carboxylic group present on the surface of ligand and hydroxyl group present on the PEG (NP-PEG-Tf). The uptake of NP-PEG-Tf into cancer cells was found to be higher as compared to non-targeted NPs. Compared with free PTX, PTX-NPs and PTX-NPs-PEG, the PTX-NPs-PEG-Tf demonstrated higher cytotoxicity to human Non-Small Cell Lung cancer cell lines (HOP-62), higher intracellular uptake especially in nuclei and lower hemolytic toxicity. Tf conjugated NPs showed increased retention time in the lungs as well as in blood. These findings indicate that Tf conjugated PEGylated nanoparticles are promising nanoconstructs for the delivery of anti-cancer drugs to cancer cells.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Antineoplastic Agents, Phytogenic administration & dosage
Antineoplastic Agents, Phytogenic chemistry
Antineoplastic Agents, Phytogenic pharmacology
Area Under Curve
Carcinoma, Non-Small-Cell Lung metabolism
Carcinoma, Non-Small-Cell Lung pathology
Cell Line, Tumor
Cell Survival drug effects
Drug Delivery Systems methods
Humans
Lung Neoplasms metabolism
Lung Neoplasms pathology
Microscopy, Fluorescence
Paclitaxel pharmacokinetics
Paclitaxel pharmacology
Particle Size
Rats
Receptors, Transferrin metabolism
Spectroscopy, Fourier Transform Infrared
Tissue Distribution
Transferrin metabolism
Chitosan chemistry
Nanoparticles chemistry
Paclitaxel chemistry
Polyethylene Glycols chemistry
Transferrin chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4367
- Volume :
- 148
- Database :
- MEDLINE
- Journal :
- Colloids and surfaces. B, Biointerfaces
- Publication Type :
- Academic Journal
- Accession number :
- 27632697
- Full Text :
- https://doi.org/10.1016/j.colsurfb.2016.08.059