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FOXO/DAF-16 Activation Slows Down Turnover of the Majority of Proteins in C. elegans.
- Source :
-
Cell reports [Cell Rep] 2016 Sep 13; Vol. 16 (11), pp. 3028-3040. - Publication Year :
- 2016
-
Abstract
- Most aging hypotheses assume the accumulation of damage, resulting in gradual physiological decline and, ultimately, death. Avoiding protein damage accumulation by enhanced turnover should slow down the aging process and extend the lifespan. However, lowering translational efficiency extends rather than shortens the lifespan in C. elegans. We studied turnover of individual proteins in the long-lived daf-2 mutant by combining SILeNCe (stable isotope labeling by nitrogen in Caenorhabditiselegans) and mass spectrometry. Intriguingly, the majority of proteins displayed prolonged half-lives in daf-2, whereas others remained unchanged, signifying that longevity is not supported by high protein turnover. This slowdown was most prominent for translation-related and mitochondrial proteins. In contrast, the high turnover of lysosomal hydrolases and very low turnover of cytoskeletal proteins remained largely unchanged. The slowdown of protein dynamics and decreased abundance of the translational machinery may point to the importance of anabolic attenuation in lifespan extension, as suggested by the hyperfunction theory.<br /> (Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cytoskeletal Proteins metabolism
Half-Life
Intracellular Membranes metabolism
Isotope Labeling
Lysosomes metabolism
Mitochondria metabolism
Muscle Proteins metabolism
Protein Biosynthesis
Proteomics
Reproducibility of Results
Stress, Physiological
Subcellular Fractions metabolism
Caenorhabditis elegans metabolism
Caenorhabditis elegans Proteins metabolism
Forkhead Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2211-1247
- Volume :
- 16
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 27626670
- Full Text :
- https://doi.org/10.1016/j.celrep.2016.07.088