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Combined IKZF1 and IG markers as new tools for diagnosis and minimal residual disease assessment in Tunisian B-ALL.
- Source :
-
Bulletin du cancer [Bull Cancer] 2016 Oct; Vol. 103 (10), pp. 822-828. Date of Electronic Publication: 2016 Sep 07. - Publication Year :
- 2016
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Abstract
- Introduction: The monitoring of minimal residual disease (MRD) approach in patients diagnosed with B-acute lymphoblastic leukemia (B-ALL) allows an early detection of residual clones inducing relapses and therefore appropriate therapy strategy. The molecular markers may identify and quantify the residual blasts in B-ALL with normal cytology. In this study, we aimed to use combined IKZF1, IGH and IGK immunoglobulin genes for diagnosis and MRD monitoring in B-ALL sample using MLPA, multiplex PCR and real-time quantitative PCR.<br />Material: We showed that multiplex PCR and MLPA are necessary and complementary to detect IKZF1 deletions.<br />Results: We have identified at the diagnosis clonal IGH rearrangement (VH3-JH5) and IKZF1 deletion (Δ4-7), which we have used it for MRD evaluation after induction chemotherapy. Despite the absence of chromosome abnormality, the patient may be classified in high-risk group with a relapse rate of residual blasts>10 <superscript>-4</superscript> and sensitivity up to 10 <superscript>-5</superscript> . This molecular approach enabled the patient's stratification, which was overlooked by classical methods.<br />Conclusion: The combined IKZF1 and immunoglobulin genes will be used as appropriate molecular tools for diagnosis and MRD assessment of B-lineage leukemias and introduced as a routine tests in Tunisian clinical laboratories. They will be useful to stratify patients into risk groups leading to better treatment strategy.<br /> (Copyright © 2016 Société Française du Cancer. Published by Elsevier Masson SAS. All rights reserved.)
- Subjects :
- Genetic Markers
Humans
Multiplex Polymerase Chain Reaction
Neoplasm, Residual
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma pathology
Gene Deletion
Genes, Immunoglobulin
Ikaros Transcription Factor genetics
Immunoglobulin Heavy Chains genetics
Immunoglobulin Light Chains genetics
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma diagnosis
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1769-6917
- Volume :
- 103
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Bulletin du cancer
- Publication Type :
- Academic Journal
- Accession number :
- 27614734
- Full Text :
- https://doi.org/10.1016/j.bulcan.2016.07.008