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Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity.
- Source :
-
Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2016; Vol. 2016, pp. 5152029. Date of Electronic Publication: 2016 Aug 17. - Publication Year :
- 2016
-
Abstract
- Creatine (Cr) is a nutritional supplement promoting a number of health benefits. Indeed Cr has been shown to be beneficial in disease-induced muscle atrophy, improve rehabilitation, and afford mild antioxidant activity. The beneficial effects are likely to derive from pleiotropic interactions. In accord with this notion, we previously demonstrated that multiple pleiotropic effects, including preservation of mitochondrial damage, account for the capacity of Cr to prevent the differentiation arrest caused by oxidative stress in C2C12 myoblasts. Given the importance of mitochondria in supporting the myogenic process, here we further explored the protective effects of Cr on the structure, function, and networking of these organelles in C2C12 cells differentiating under oxidative stressing conditions; the effects on the energy sensor AMPK, on PGC-1α, which is involved in mitochondrial biogenesis and its downstream effector Tfam were also investigated. Our results indicate that damage to mitochondria is crucial in the differentiation imbalance caused by oxidative stress and that the Cr-prevention of these injuries is invariably associated with the recovery of the normal myogenic capacity. We also found that Cr activates AMPK and induces an upregulation of PGC-1α expression, two events which are likely to contribute to the protection of mitochondrial quality and function.
- Subjects :
- AMP-Activated Protein Kinases metabolism
Animals
Cell Line
Cytoprotection
DNA, Mitochondrial drug effects
DNA, Mitochondrial genetics
DNA, Mitochondrial metabolism
DNA-Binding Proteins metabolism
Enzyme Activation
High Mobility Group Proteins metabolism
Hydrogen Peroxide toxicity
Lipid Peroxidation drug effects
Membrane Potential, Mitochondrial drug effects
Mice
Mitochondria, Muscle metabolism
Mitochondria, Muscle ultrastructure
Myoblasts, Skeletal metabolism
Myoblasts, Skeletal ultrastructure
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha metabolism
Proteomics methods
Signal Transduction drug effects
Cell Differentiation drug effects
Creatine pharmacology
Mitochondria, Muscle drug effects
Muscle Development drug effects
Myoblasts, Skeletal drug effects
Oxidative Stress drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1942-0994
- Volume :
- 2016
- Database :
- MEDLINE
- Journal :
- Oxidative medicine and cellular longevity
- Publication Type :
- Academic Journal
- Accession number :
- 27610211
- Full Text :
- https://doi.org/10.1155/2016/5152029