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MRI Dynamically Evaluates the Therapeutic Effect of Recombinant Human MANF on Ischemia/Reperfusion Injury in Rats.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2016 Sep 05; Vol. 17 (9). Date of Electronic Publication: 2016 Sep 05. - Publication Year :
- 2016
-
Abstract
- As an endoplasmic reticulum (ER) stress-inducible protein, mesencephalic astrocyte-derived neurotrophic factor (MANF) has been proven to protect dopaminergic neurons and nondopaminergic cells. Our previous studies had shown that MANF protected against ischemia/reperfusion injury. Here, we developed a magnetic resonance imaging (MRI) technology to dynamically evaluate the therapeutic effects of MANF on ischemia/reperfusion injury. We established a rat focal ischemic model by using middle cerebral artery occlusion (MCAO). MRI was performed to investigate the dynamics of lesion formation. MANF protein was injected into the right lateral ventricle at 3 h after reperfusion following MCAO for 90 min, when the obvious lesion firstly appeared according to MRI investigation. T2-weighted imaging for evaluating the therapeutic effects of MANF protein was performed in ischemia/reperfusion injury rats on Days 1, 2, 3, 5, and 7 post-reperfusion combined with histology methods. The results indicated that the administration of MANF protein at the early stage after ischemia/reperfusion injury decreased the mortality, improved the neurological function, reduced the cerebral infarct volume, and alleviated the brain tissue injury. The findings collected from MRI are consistent with the morphological and pathological changes, which suggest that MRI is a useful technology for evaluating the therapeutic effects of drugs.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Animals
Brain blood supply
Brain diagnostic imaging
Humans
Infarction, Middle Cerebral Artery diagnostic imaging
Male
Nerve Growth Factors administration & dosage
Rats
Rats, Sprague-Dawley
Recombinant Proteins
Reperfusion Injury diagnostic imaging
Infarction, Middle Cerebral Artery drug therapy
Magnetic Resonance Imaging
Nerve Growth Factors therapeutic use
Reperfusion Injury drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 17
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 27608005
- Full Text :
- https://doi.org/10.3390/ijms17091476