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cAMP-dependent recruitment of acidic organelles for Ca2+ signaling in the salivary gland.
- Source :
-
American journal of physiology. Cell physiology [Am J Physiol Cell Physiol] 2016 Nov 01; Vol. 311 (5), pp. C697-C709. Date of Electronic Publication: 2016 Sep 07. - Publication Year :
- 2016
-
Abstract
- Autonomic neural activation of intracellular Ca <superscript>2+</superscript> release in parotid acinar cells induces the secretion of the fluid and protein components of primary saliva critical for maintaining overall oral homeostasis. In the current study, we profiled the role of acidic organelles in shaping the Ca <superscript>2+</superscript> signals of parotid acini using a variety of imaging and pharmacological approaches. Results demonstrate that zymogen granules predominate as an apically polarized population of acidic organelles that contributes to the initial Ca <superscript>2+</superscript> release. Moreover, we provide evidence that indicates a role for the intracellular messenger NAADP in the release of Ca <superscript>2+</superscript> from acidic organelles following elevation of cAMP. Our data are consistent with the "trigger" hypothesis where localized release of Ca <superscript>2+</superscript> sensitizes canonical intracellular Ca <superscript>2+</superscript> channels to enhance signals from the endoplasmic reticulum. Release from acidic stores may be important for initiating saliva secretion at low levels of stimulation and a potential therapeutic target to augment secretory activity in hypofunctioning salivary glands.<br /> (Copyright © 2016 the American Physiological Society.)
- Subjects :
- Acinar Cells metabolism
Animals
Calcium Channels metabolism
Endoplasmic Reticulum metabolism
Male
Mice
Mice, Inbred C57BL
NADP analogs & derivatives
NADP metabolism
Parotid Gland metabolism
Secretory Vesicles metabolism
Acids metabolism
Calcium metabolism
Calcium Signaling physiology
Cyclic AMP metabolism
Salivary Glands metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1522-1563
- Volume :
- 311
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Cell physiology
- Publication Type :
- Academic Journal
- Accession number :
- 27605449
- Full Text :
- https://doi.org/10.1152/ajpcell.00010.2016