Back to Search
Start Over
Advanced and High-Throughput Method for Mitochondrial Bioenergetics Evaluation in Neurotrauma.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2016; Vol. 1462, pp. 597-610. - Publication Year :
- 2016
-
Abstract
- Mitochondrial dysfunction is one of the key posttraumatic neuropathological events observed in various experimental models of traumatic brain injury (TBI). The extent of mitochondrial dysfunction has been associated with the severity and time course of secondary injury following brain trauma. Critically, several mitochondrial targeting preclinical drugs used in experimental TBI models have shown improved mitochondrial bioenergetics, together with cortical tissue sparing and cognitive behavioral outcome. Mitochondria, being a central regulator of cellular metabolic pathways and energy producer of cells, are of a great interest for researchers aiming to adopt cutting-edge methodology for mitochondrial bioenergetics assessment. The traditional way of mitochondrial bioenergetics analysis utilizing a Clark-type oxygen electrode (aka. oxytherm) is time-consuming and labor-intensive. In the present chapter, we describe an advanced and high-throughput method for mitochondrial bioenergetics assessments utilizing the Seahorse Biosciences XF(e)24 Flux Analyzer. This allows for simultaneous measurement of multiple samples with higher efficiency than the oxytherm procedure. This chapter provides helpful guidelines for conducting mitochondrial isolation and studying mitochondrial bioenergetics in brain tissue homogenates following experimental TBI.
- Subjects :
- Animals
Cell Respiration drug effects
Drug Discovery
Drug Evaluation, Preclinical
Male
Metabolomics methods
Mitochondria drug effects
Oxidative Phosphorylation
Rats
Reactive Oxygen Species metabolism
Trauma, Nervous System drug therapy
Energy Metabolism
High-Throughput Screening Assays
Mitochondria metabolism
Trauma, Nervous System etiology
Trauma, Nervous System metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1462
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 27604740
- Full Text :
- https://doi.org/10.1007/978-1-4939-3816-2_32