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Growth Hormone differentially modulates chemoresistance in human endometrial adenocarcinoma cell lines.
- Source :
-
Endocrine [Endocrine] 2017 Jun; Vol. 56 (3), pp. 621-632. Date of Electronic Publication: 2016 Sep 01. - Publication Year :
- 2017
-
Abstract
- Growth Hormone may influence neoplastic development of endometrial epithelium towards endometrial adenocarcinoma, which is one of the most occurring tumors in acromegalic patients. Since chemoresistance often develops in advanced endometrial adenocarcinoma, we investigated whether Growth Hormone might influence the development of chemoresistance to drugs routinely employed in endometrial adenocarcinoma treatment, such as Doxorubicin, Cisplatin, and Paclitaxel. Growth Hormone and Growth Hormone receptor expression was assessed by immunofluorescence in two endometrial adenocarcinoma cell lines, AN3 CA and HEC-1-A cells. Growth Hormone effects were assessed investigating cell viability, caspase3/7 activation, ERK1/2, and protein kinase C delta protein expression. AN3 CA and HEC-1-A cells display Growth Hormone and Growth Hormone receptor. Growth Hormone does not influence cell viability in both cells lines, but significantly reduces caspase 3/7 activation in AN3 CA cells, an effect blocked by a Growth Hormone receptor antagonist. Growth Hormone rescues AN3 CA cells from the inhibitory effects of Doxorubicin and Cisplatin on cell viability, while it has no effect on Paclitaxel. Growth Hormone does not influence the pro-apoptotic effects of Doxorubicin, but is capable of rescuing AN3 CA cells from the pro-apoptotic effects of Cisplatin. On the other hand, Growth Hormone did not influence the effects of Doxorubicin and Paclitaxel on HEC-1A cell viability. The protective action of Growth Hormone towards the effects of Doxorubicin may be mediated by ERK1/2 activation, while the pro-apoptotic effects of Cisplatin may be mediated by protein kinase C delta inhibition. All together our results indicate that Growth Hormone may differentially contribute to endometrial adenocarcinoma chemoresistance. This may provide new insights on novel therapies against endometrial adenocarcinoma chemoresistant aggressive tumors.
- Subjects :
- Adenocarcinoma metabolism
Adenocarcinoma pathology
Antineoplastic Agents therapeutic use
Antineoplastic Agents, Hormonal pharmacology
Antineoplastic Agents, Hormonal therapeutic use
Caspase 3 metabolism
Caspase 7 metabolism
Cell Line, Tumor
Cisplatin therapeutic use
Doxorubicin therapeutic use
Drug Resistance, Neoplasm
Endometrial Neoplasms metabolism
Endometrial Neoplasms pathology
Female
Human Growth Hormone metabolism
Human Growth Hormone pharmacology
Humans
Nuclear Export Signals drug effects
Paclitaxel pharmacology
Paclitaxel therapeutic use
Receptors, Somatotropin metabolism
Adenocarcinoma drug therapy
Antineoplastic Agents pharmacology
Apoptosis drug effects
Cell Survival drug effects
Cisplatin pharmacology
Doxorubicin pharmacology
Endometrial Neoplasms drug therapy
Human Growth Hormone analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1559-0100
- Volume :
- 56
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Endocrine
- Publication Type :
- Academic Journal
- Accession number :
- 27585662
- Full Text :
- https://doi.org/10.1007/s12020-016-1085-4