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Processing of Structurally Heterogeneous Cryo-EM Data in RELION.
- Source :
-
Methods in enzymology [Methods Enzymol] 2016; Vol. 579, pp. 125-57. Date of Electronic Publication: 2016 May 31. - Publication Year :
- 2016
-
Abstract
- This chapter describes algorithmic advances in the RELION software, and how these are used in high-resolution cryo-electron microscopy (cryo-EM) structure determination. Since the presence of projections of different three-dimensional structures in the dataset probably represents the biggest challenge in cryo-EM data processing, special emphasis is placed on how to deal with structurally heterogeneous datasets. As such, this chapter aims to be of practical help to those who wish to use RELION in their cryo-EM structure determination efforts.<br /> (© 2016 Elsevier Inc. All rights reserved.)
- Subjects :
- Bacterial Proteins ultrastructure
Dose-Response Relationship, Radiation
Escherichia coli ultrastructure
Image Processing, Computer-Assisted statistics & numerical data
Imaging, Three-Dimensional instrumentation
Imaging, Three-Dimensional methods
Plasmodium falciparum ultrastructure
Protein Conformation
Ribosomes ultrastructure
beta-Galactosidase ultrastructure
Algorithms
Cryoelectron Microscopy methods
Image Processing, Computer-Assisted methods
Software
Subjects
Details
- Language :
- English
- ISSN :
- 1557-7988
- Volume :
- 579
- Database :
- MEDLINE
- Journal :
- Methods in enzymology
- Publication Type :
- Academic Journal
- Accession number :
- 27572726
- Full Text :
- https://doi.org/10.1016/bs.mie.2016.04.012