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Sequential and sustained release of SDF-1 and BMP-2 from silk fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration.
- Source :
-
Biomaterials [Biomaterials] 2016 Nov; Vol. 106, pp. 205-16. Date of Electronic Publication: 2016 Aug 17. - Publication Year :
- 2016
-
Abstract
- In this study, a cell-free bone tissue engineering system based on a silk fibroin (SF)/nano-hydroxyapatite (nHAp) scaffold was developed, in which two bioactive molecules, stromal cell derived factor-1 (SDF-1) and bone morphogenetic protein-2 (BMP-2), were embedded and released in a sequential and controlled manner to facilitate cell recruitment and bone formation, respectively. BMP-2 was initially loaded into SF microspheres, and these BMP-2 containing microspheres were subsequently encapsulated into the SF/nHAp scaffolds, which were successively functionalized with SDF-1 via physical adsorption. The results indicated rapid initial release of SDF-1 during the first few days, followed by slow and sustained release of BMP-2 for as long as three weeks. The composite scaffold significantly promoted the recruitment of bone marrow mesenchymal stem cells (BMSCs) and osteogenic differentiation of them in vitro. Further, the in vivo studies using D-Luciferin-labeled BMSCs indicated that implantation of this composite scaffold markedly promoted the recruitment of BMSCs to the implanted sites. Enhanced bone regeneration was identified at 12 weeks' post-implantation. Taken together, our findings suggested that the sequential and sustained release of SDF-1 and BMP-2 from the SF/nHAp scaffolds resulted in a synergistic effect on bone regeneration. Such a composite system, therefore, shows promising potential for cell-free bone tissue engineering applications.<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Bone Regeneration drug effects
Bone Regeneration physiology
Dose-Response Relationship, Drug
Drug Implants administration & dosage
Drug Implants chemistry
Male
Mesenchymal Stem Cells cytology
Mesenchymal Stem Cells physiology
Osteoblasts cytology
Osteoblasts physiology
Osteogenesis physiology
Rats
Rats, Sprague-Dawley
Bone Morphogenetic Protein 2 administration & dosage
Chemokine CXCL12 administration & dosage
Durapatite chemistry
Fibroins chemistry
Nanoparticles chemistry
Skull Fractures therapy
Tissue Scaffolds
Subjects
Details
- Language :
- English
- ISSN :
- 1878-5905
- Volume :
- 106
- Database :
- MEDLINE
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 27566869
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2016.08.023