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Secretome proteomics reveals candidate non-invasive biomarkers of BRCA1 deficiency in breast cancer.
- Source :
-
Oncotarget [Oncotarget] 2016 Sep 27; Vol. 7 (39), pp. 63537-63548. - Publication Year :
- 2016
-
Abstract
- Breast cancer arising in female BRCA1 mutation carriers is characterized by an aggressive phenotype and early age of onset. We performed tandem mass spectrometry-based proteomics of secretomes and exosome-like extracellular vesicles from BRCA1-deficient and BRCA1-proficient murine breast tumor models to identify extracellular protein biomarkers, which can be used as an adjunct to current diagnostic modalities in patients with BRCA1-deficient breast cancer. We identified 2,107 proteins, of which 215 were highly enriched in the BRCA1-deficient secretome. We demonstrated that BRCA1-deficient secretome proteins could cluster most human BRCA1- and BRCA2-related breast carcinomas at the transcriptome level. Topoisomerase I (TOP1) and P-cadherin (CDH3) expression was investigated by immunohistochemistry on tissue microarrays of a large panel of 253 human breast carcinomas with and without BRCA1/2 mutations. We showed that expression of TOP1 and CDH3 was significantly increased in human BRCA1-related breast carcinomas relative to sporadic cases (p = 0.002 and p < 0.001, respectively). Multiple logistic regression showed that TOP1 (adjusted odds ratio [OR] 3.75; 95% confidence interval [95% CI], 1.85 - 7.71, p < 0.001) as well as CDH3 positivity (adjusted OR 2.45; 95% CI, 1.08 - 5.49, p = 0.032) were associated with BRCA1/2-related breast carcinomas after adjustment for triple-negative phenotype and age. In conclusion, proteome profiling of secretome using murine breast tumor models is a powerful strategy to identify non-invasive candidate biomarkers of BRCA1-deficient breast cancer. We demonstrate that TOP1 and CDH3 are closely associated to BRCA1-deficient breast cancer. These data merit further investigation for early detection of tumors arising in BRCA1 mutation carriers.
- Subjects :
- Animals
BRCA1 Protein genetics
BRCA2 Protein deficiency
BRCA2 Protein genetics
Breast Neoplasms genetics
Breast Neoplasms pathology
Carcinoma, Ductal, Breast genetics
Carcinoma, Ductal, Breast pathology
Carcinoma, Lobular genetics
Carcinoma, Lobular pathology
Carcinoma, Medullary genetics
Carcinoma, Medullary pathology
Disease Models, Animal
Female
Humans
Mice
Middle Aged
Mutation
Proteomics methods
Tumor Cells, Cultured
BRCA1 Protein deficiency
Biomarkers, Tumor metabolism
Breast Neoplasms metabolism
Carcinoma, Ductal, Breast metabolism
Carcinoma, Lobular metabolism
Carcinoma, Medullary metabolism
Proteome analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1949-2553
- Volume :
- 7
- Issue :
- 39
- Database :
- MEDLINE
- Journal :
- Oncotarget
- Publication Type :
- Academic Journal
- Accession number :
- 27566577
- Full Text :
- https://doi.org/10.18632/oncotarget.11535