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Effects of Etomidate on GABAergic and Glutamatergic Transmission in Rat Thalamocortical Slices.
- Source :
-
Neurochemical research [Neurochem Res] 2016 Dec; Vol. 41 (12), pp. 3181-3191. Date of Electronic Publication: 2016 Aug 26. - Publication Year :
- 2016
-
Abstract
- Although accumulative evidence indicates that the thalamocortical system is an important target for general anesthetics, the underlying mechanisms of anesthetic action on thalamocortical neurotransmission are not fully understood. The aim of the study is to explore the action of etomidate on glutamatergic and GABAergic transmission in rat thalamocortical slices by using whole cell patch-clamp recording. We found that etomidate mainly prolonged the decay time of spontaneous GABAergic inhibitory postsynaptic currents (sIPSCs), without changing the frequency. Furthermore, etomidate not only prolonged the decay time of miniature inhibitory postsynaptic currents (mIPSCs) but also increased the amplitude. On the other hand, etomidate significantly decreased the frequency of spontaneous glutamatergic excitatory postsynaptic currents (sEPSCs), without altering the amplitude or decay time in the absence of bicuculline. When GABA <subscript>A</subscript> receptors were blocked using bicuculline, the effects of etomidate on sEPSCs were mostly eliminated. These results suggest that etomidate enhances GABAergic transmission mainly through postsynaptic mechanism in thalamocortical neuronal network. Etomidate attenuates glutamatergic transmission predominantly through presynaptic action and requires presynaptic GABA <subscript>A</subscript> receptors involvement.
- Subjects :
- Animals
Bicuculline pharmacology
Cerebral Cortex physiology
Excitatory Postsynaptic Potentials drug effects
GABA-A Receptor Antagonists pharmacology
In Vitro Techniques
Inhibitory Postsynaptic Potentials drug effects
Miniature Postsynaptic Potentials drug effects
Rats, Sprague-Dawley
Receptors, GABA-A physiology
Receptors, Presynaptic physiology
Synaptic Transmission drug effects
Thalamus physiology
Anesthetics, General pharmacology
Cerebral Cortex drug effects
Etomidate pharmacology
Glutamic Acid physiology
Thalamus drug effects
gamma-Aminobutyric Acid physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1573-6903
- Volume :
- 41
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Neurochemical research
- Publication Type :
- Academic Journal
- Accession number :
- 27561291
- Full Text :
- https://doi.org/10.1007/s11064-016-2042-6