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Expression of EIF5A2 associates with poor survival of nasopharyngeal carcinoma patients treated with induction chemotherapy.
- Source :
-
BMC cancer [BMC Cancer] 2016 Aug 22; Vol. 16, pp. 669. Date of Electronic Publication: 2016 Aug 22. - Publication Year :
- 2016
-
Abstract
- Background: Nasopharyngeal carcinoma (NPC) is a type of head-neck cancer with a distinguishable geographic and racial distribution worldwide. Increasing evidence supports that the accumulation of additional genetic and epigenetic abnormalities is important in driving the NPC tumorigenic process. In this study, we aim to investigate the association between EIF5A2 (Eukaryotic translation initiation factor 5A2) expression status and NPC clinical outcomes.<br />Methods: The expression status of EIF5A2 was investigated in the NPC tissue microarray. Tissues were from 166 NPC patients staging II-IV, collected between 1999 and 2005. All patients were administered 2-3 cycles of DDP (cisplatin) + 5-Fu (5-fluorouracil) induction therapy and then treated with a uniform conventional two-dimensional radiotherapy. Cell motility assay, tumor growth assay and cytotoxicity assay were performed on the EIF5A2 overexpressed cells and control cells. siRNA was also used in the in vitro studies.<br />Results: Positive staining of EIF5A2 was observed in 85.4 % (105/123) informative tumor cases. Multivariate analyses demonstrated that EIF5A2 was an independent prognostic marker of poor overall survival (OS) (P = 0.041), failure-free survival (FFS) (P = 0.029), and distant failure-free survival (D-FFS) (P = 0.043) in patients with locoregionally advanced NPC patients treated with cisplatin + 5-Fu chemoradiotherapy. The forced expression of EIF5A2 in NPC cells enhanced the cells' motility and growth ability. Knock-down of EIF5A2 in NPC cells decreased the cell's motility and growth ability. Our results also demonstrated that EIF5A2 overexpression induced chemoresistance of NPC cells to 5-Fu.<br />Conclusions: Our findings suggested that EIF5A2 expression, as examined by immunohistochemistry, could function as an independent prognostic factor of outcomes in NPC patients with cisplatin + 5-Fu chemoradiotherapy. EIF5A2 might be a novel therapeutic target for the inhibition of NPC progress.
- Subjects :
- Adult
Aged
Biomarkers, Tumor genetics
Carcinoma genetics
Cell Line, Tumor
Cell Movement genetics
Cell Proliferation
Chemoradiotherapy methods
Cisplatin therapeutic use
Female
Fluorouracil therapeutic use
Humans
Male
Middle Aged
Nasopharyngeal Carcinoma
Nasopharyngeal Neoplasms genetics
Peptide Initiation Factors genetics
RNA Interference
RNA, Small Interfering genetics
RNA-Binding Proteins genetics
Young Adult
Eukaryotic Translation Initiation Factor 5A
Carcinoma drug therapy
Carcinoma mortality
Induction Chemotherapy methods
Nasopharyngeal Neoplasms drug therapy
Nasopharyngeal Neoplasms mortality
Peptide Initiation Factors biosynthesis
RNA-Binding Proteins biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2407
- Volume :
- 16
- Database :
- MEDLINE
- Journal :
- BMC cancer
- Publication Type :
- Academic Journal
- Accession number :
- 27549330
- Full Text :
- https://doi.org/10.1186/s12885-016-2714-2