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Y-box-binding protein 1 as a non-canonical factor of base excision repair.
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 2016 Dec; Vol. 1864 (12), pp. 1631-1640. Date of Electronic Publication: 2016 Aug 18. - Publication Year :
- 2016
-
Abstract
- Base excision repair (BER) is a flagship DNA repair system responsible for maintaining genome integrity. Apart from basal enzymes, this system involves several accessory factors essential for coordination and regulation of DNA processing during substrate channeling. Y-box-binding protein 1 (YB-1), a multifunctional factor that can interact with DNA, RNA, poly(ADP-ribose) and plenty of proteins including DNA repair enzymes, is increasingly considered as a non-canonical protein of BER. Here we provide quantitative characterization of YB-1 physical interactions with key BER factors such as PARP1, PARP2, APE1, NEIL1 and pol β and comparison of the full-length YB-1 and its C-terminally truncated nuclear form in regard to their binding affinities for BER proteins. Data on functional interactions reveal strong stimulation of PARP1 autopoly(ADP-ribosyl)ation and inhibition of poly(ADP-ribose) degradation by PARG in the presence of YB-1. Moreover, YB-1 is shown to stimulate AP lyase activity of NEIL1 and to inhibit dRP lyase activity of pol β on model DNA duplex structure. We also demonstrate for the first time YB-1 poly(ADP-ribosyl)ation in the presence of RNA.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
DNA Damage
DNA Glycosylases metabolism
DNA Polymerase beta metabolism
DNA-(Apurinic or Apyrimidinic Site) Lyase chemistry
DNA-(Apurinic or Apyrimidinic Site) Lyase genetics
DNA-(Apurinic or Apyrimidinic Site) Lyase metabolism
Humans
Mice
Peptide Fragments chemistry
Peptide Fragments genetics
Peptide Fragments metabolism
Poly (ADP-Ribose) Polymerase-1 genetics
Poly (ADP-Ribose) Polymerase-1 metabolism
Poly(ADP-ribose) Polymerases genetics
Poly(ADP-ribose) Polymerases metabolism
Protein Interaction Domains and Motifs
Recombinant Proteins chemistry
Recombinant Proteins genetics
Recombinant Proteins metabolism
Spectrometry, Fluorescence
Y-Box-Binding Protein 1 chemistry
Y-Box-Binding Protein 1 genetics
DNA Repair physiology
Y-Box-Binding Protein 1 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 1864
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 27544639
- Full Text :
- https://doi.org/10.1016/j.bbapap.2016.08.012