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[Construction of Protein Phosphatase 2A Catalytic Subunit β (PPP2Cβ) Overexpression Lentiviral Vector and Its Effect on K562 Erythroid Differentiation].

Authors :
Li M
Zhao K
Dong XM
Zhan YQ
Yin RH
Yang XM
Li CY
Source :
Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2016 Aug; Vol. 24 (4), pp. 1173-8.
Publication Year :
2016

Abstract

Objective: To construct the ovexpression lentivirus vector of PPP2Cβ, the catalytic subunit of protein phosphatase 2A, so as to obtain high-titer packaged lentivirus particles, and to examine the effect of PPP2Cβ on the erythroid differentiation Methods: The CDS of PPP2Cβ was cloned into the second generation of lentivirus vector FUGW, which should be used to co-transfect HEK 293T cells with the lentiviral expression vector and packaging vectors including pMD2G and pSPAX2. Lentiviruses were harvested at 36 and 48 hours after transfection. Titers of viral stock were determined by using flow cytometric analysis. The Western blot was performed to detect the expression level of PPP2Cβ in K562 cells transinfected with the lentiviruses. Benzidine staining and real-time PCR analysis were used to assess the erythroid differentiation of K562 cells.<br />Results: The PPP2Cβ overexpressing lentivirus vectors were constructed, the high-titer lentiviral particles were obtained, and then the PPP2Cβ overexpression K562 cell line was established and promote erythroid differentiation of K562 cells.<br />Conclusion: This study suggests that overexpression PPP2Cβ can promote K562 cell erythroid differentiation.

Details

Language :
Chinese
ISSN :
1009-2137
Volume :
24
Issue :
4
Database :
MEDLINE
Journal :
Zhongguo shi yan xue ye xue za zhi
Publication Type :
Academic Journal
Accession number :
27531795
Full Text :
https://doi.org/10.7534/j.issn.1009-2137.2016.04.039