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KDM6B histone demethylase is an epigenetic regulator of estrogen receptor β expression in human pleural mesothelioma.
- Source :
-
Epigenomics [Epigenomics] 2016 Sep; Vol. 8 (9), pp. 1227-38. Date of Electronic Publication: 2016 Aug 16. - Publication Year :
- 2016
-
Abstract
- Aim: To assess the correlation between KDM6B and estrogen receptor β (ERβ) expression in malignant pleural mesothelioma (MPM).<br />Materials & Methods: We evaluated gene expression by in silico analysis of microarray data, real-time PCR and western blot in MPM tumors and cell lines.<br />Results & Conclusion: We report a strong positive correlation between the expression of KDM6B and ERβ in MPM tumors and cell lines. We describe that, in hypoxia, the HIF2α-KDM6B axis induces an epithelioid morphology and ERβ expression in biphasic MPM cells with estrogen receptor-negative phenotype. Reduced histone H3K27 tri-methylation confirms KDM6B activity under hypoxic conditions. Importantly, cells treated during reoxygenation with the selective ERβ agonist, KB9520, maintain ERβ expression and the less aggressive phenotype acquired in hypoxia.
- Subjects :
- Basic Helix-Loop-Helix Transcription Factors metabolism
Cell Hypoxia
Cell Line, Tumor
Estrogen Receptor beta metabolism
Histones metabolism
Humans
Jumonji Domain-Containing Histone Demethylases metabolism
Lung Neoplasms metabolism
Mesothelioma metabolism
Mesothelioma, Malignant
Methylation
Protein Processing, Post-Translational
Epigenesis, Genetic
Estrogen Receptor beta genetics
Jumonji Domain-Containing Histone Demethylases genetics
Lung Neoplasms genetics
Mesothelioma genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1750-192X
- Volume :
- 8
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Epigenomics
- Publication Type :
- Academic Journal
- Accession number :
- 27529370
- Full Text :
- https://doi.org/10.2217/epi-2016-0025