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Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2016; Vol. 1474, pp. 171-85. - Publication Year :
- 2016
-
Abstract
- Two-photon (2P) imaging has proven to be a powerful tool for investigating neural structure and function both in brain slices and in intact systems. In vivo 2P imaging presents significant challenges in sample preparation, which are exacerbated in non-murine species. Here, we describe procedures for the effective virally mediated labeling of neurons and for the implantation of cranial windows for imaging. The procedures described here are applicable to a range of species, including mice, and are routinely used in ferrets and tree shrews to provide large-scale labeling of cortical volumes and high-quality imaging data.
- Subjects :
- Animals
Calcium metabolism
Calcium Signaling
Craniotomy methods
Dependovirus metabolism
Ferrets
Genetic Vectors chemistry
Genetic Vectors metabolism
Mice
Neocortex metabolism
Neurons metabolism
Stereotaxic Techniques
Tupaiidae
Visual Cortex metabolism
Dependovirus genetics
Microscopy, Fluorescence, Multiphoton methods
Neocortex ultrastructure
Neurons ultrastructure
Skull surgery
Visual Cortex ultrastructure
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1474
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 27515080
- Full Text :
- https://doi.org/10.1007/978-1-4939-6352-2_10