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A self-sufficient system for removal of synthetic dye by coupling of spore-displayed triphenylmethane reductase and glucose 1-dehydrogenase.
- Source :
-
Environmental science and pollution research international [Environ Sci Pollut Res Int] 2016 Nov; Vol. 23 (21), pp. 21319-21326. Date of Electronic Publication: 2016 Aug 08. - Publication Year :
- 2016
-
Abstract
- Biodegradation of triphenylmethane dyes by microorganisms is hampered by the transport barrier imposed by cell membranes. On the other hand, cell-free systems using enzyme-based biodegradation strategy are costly. Therefore, an efficient and inexpensive approach circumventing these problems is highly desirable. Here, we constructed a self-sufficient system for synthetic dye removal by coupling of spore surface-displayed triphenylmethane reductase (TMR) and glucose 1-dehydrogenase (GDH) for the first time. Display of both TMR and GDH significantly enhanced their stability under conditions of extreme pH and temperature. These engineered spores also exhibited more robust long-term stability than their purified counterparts. Furthermore, we observed that a high ratio of spore-displayed GDH is necessary for high dye degradation efficiency. These results indicate that this continuous dye removal system with cofactor regeneration offers a promising solution for dye biodegradation applications.
- Subjects :
- Biodegradation, Environmental
Environmental Pollutants isolation & purification
Environmental Pollutants metabolism
Glucose 1-Dehydrogenase metabolism
Temperature
Bacillus subtilis metabolism
Coloring Agents isolation & purification
Coloring Agents metabolism
Oxidoreductases isolation & purification
Oxidoreductases metabolism
Spores, Bacterial metabolism
Trityl Compounds metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1614-7499
- Volume :
- 23
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Environmental science and pollution research international
- Publication Type :
- Academic Journal
- Accession number :
- 27502455
- Full Text :
- https://doi.org/10.1007/s11356-016-7330-9