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The Necessity of OCT-4 and CDX2 for Early Development and Gene Expression Involved in Differentiation of Inner Cell Mass and Trophectoderm Lineages in Bovine Embryos.
- Source :
-
Cellular reprogramming [Cell Reprogram] 2016 Oct; Vol. 18 (5), pp. 309-318. Date of Electronic Publication: 2016 Aug 08. - Publication Year :
- 2016
-
Abstract
- The functions of POU class 5 transcription factor 1 (Oct-4) and caudal-type homeobox 2 (Cdx2) in the differentiation of the murine inner cell mass (ICM) and trophectoderm (TE) have been described in detail. However, little is known about the roles of OCT-4 and CDX2 in preimplantation bovine embryos. To elucidate their functions during early development in bovine embryos, we performed OCT-4 and CDX2 downregulation using RNA interference. We injected OCT-4- or CDX2-specific short interfering RNAs (siRNAs) into bovine zygotes. The rate of blastocyst development of OCT-4-downregulated embryos was lower compared with uninjected or control siRNA-injected embryos. Gene expression analysis revealed decreased CDX2 and fibroblast growth factor 4 expression in OCT-4-downregulated embryos. CDX2-downregulated embryos developed to the blastocyst stage; however, in most cases, blastocoel formation was delayed. Gene expression analysis revealed decreased GATA3 expression and elevated NANOG expression in CDX2-downregulated embryos. In conclusion, OCT-4 and CDX2 are essential for early development and gene expression involved in differentiation of ICM and TE lineages in bovine embryos.
- Subjects :
- Animals
Blastocyst Inner Cell Mass metabolism
CDX2 Transcription Factor genetics
Cattle
Cells, Cultured
Embryo, Mammalian metabolism
Embryonic Development
Female
Gene Expression Regulation, Developmental
Octamer Transcription Factor-3 genetics
Trophoblasts metabolism
Blastocyst Inner Cell Mass cytology
CDX2 Transcription Factor metabolism
Cell Differentiation
Cell Lineage genetics
Embryo, Mammalian cytology
Octamer Transcription Factor-3 metabolism
Trophoblasts cytology
Subjects
Details
- Language :
- English
- ISSN :
- 2152-4998
- Volume :
- 18
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Cellular reprogramming
- Publication Type :
- Academic Journal
- Accession number :
- 27500421
- Full Text :
- https://doi.org/10.1089/cell.2015.0081