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Paper-based fluorescent sensor for rapid naked-eye detection of acetylcholinesterase activity and organophosphorus pesticides with high sensitivity and selectivity.
- Source :
-
Biosensors & bioelectronics [Biosens Bioelectron] 2016 Dec 15; Vol. 86, pp. 971-977. Date of Electronic Publication: 2016 Jul 12. - Publication Year :
- 2016
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Abstract
- Various strategies have been proposed for the sensing of acetylcholinesterase (AChE) activity and organophosphorus pesticides (OPs). However, the practical application of most methods is restricted by their intrinsic drawbacks such as complexity, long analysis time, and high cost. Thus, it is highly desirable to develop simple, fast and sensitive approaches for AChE activity and OPs detection. Herein, we reported a simple paper-based fluorescent sensor (PFS) based on the aggregation induced emission (AIE) effect of tetraphenylethylene (TPE) and the addition reaction capability of maleimide, which has been used as a powerful tool for rapid naked-eye detection of AChE activity and OPs. The introduction of TPE provides the probe with unique fluorescence property in solid state and is of great importance for improving the sensitivity of PFS. The hydrolysis product of acetylthiocholine catalyzed by AChE induced the maleimide ring destruction and activated the fluorescence performance of TPE. Given that AChE activity can be specifically inhibited by OPs, the as-proposed PFS can also be utilized for sensitive detection of OPs. Meanwhile, the variation of fluorescence signal can be readily detected by naked eyes, and low detection limits of 2.5mUmL(-1) and 0.5ngmL(-1) for AChE activity and OPs are obtained, respectively. Moreover, it has been successfully applied for AChE activity and OPs detection in diluted human serum samples, showing its great potential to be applied in real samples. Thus, this strategy possesses considerable advantages of simplicity, rapid detection, portability, cost efficiency and visualization.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- Acetylcholinesterase analysis
Disposable Equipment
Enzyme Activation
Equipment Design
Equipment Failure Analysis
Organophosphorus Compounds chemistry
Pesticides chemistry
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Fluorescence instrumentation
Acetylcholinesterase chemistry
Colorimetry instrumentation
Environmental Monitoring instrumentation
Organophosphorus Compounds analysis
Paper
Pesticides analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4235
- Volume :
- 86
- Database :
- MEDLINE
- Journal :
- Biosensors & bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 27498323
- Full Text :
- https://doi.org/10.1016/j.bios.2016.07.022