Back to Search Start Over

Serological Analysis of Immunogenic Properties of Recombinant Meningococcus IgA1 Protease-Based Proteins.

Serological Analysis of Immunogenic Properties of Recombinant Meningococcus IgA1 Protease-Based Proteins.

Authors :
Kotelnikova OV
Zinchenko AA
Vikhrov AA
Alliluev AP
Serova OV
Gordeeva EA
Zhigis LS
Zueva VS
Razgulyaeva OA
Melikhova TD
Nokel EA
Drozhzhina EY
Rumsh LD
Source :
Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2016 Jul; Vol. 161 (3), pp. 391-4. Date of Electronic Publication: 2016 Aug 06.
Publication Year :
2016

Abstract

Using the genome sequence of IgA1 protease of N. meningitidis of serogroup B, four recombinant proteins of different structure and molecular weight were constructed. These proteins were equal in inducing the formation of specific antibodies to IgA1 protease and had protective properties against meningococci. In the sera of immunized mice, anti-IgA1 protease antibodies were detected by whole-cell ELISA, which indicated the presence of IgA1 protease on the surface of these bacteria. We hypothesized that the protective properties of IgA1 protease-based antigens and IgA1 protease analogs could be realized not only via impairment of bacterium adhesion to the mucosa, but also via suppression of this pathogen in the organism. The presented findings seem promising for using these proteins as the basis for anti-meningococcus vaccine.

Details

Language :
English
ISSN :
1573-8221
Volume :
161
Issue :
3
Database :
MEDLINE
Journal :
Bulletin of experimental biology and medicine
Publication Type :
Academic Journal
Accession number :
27496029
Full Text :
https://doi.org/10.1007/s10517-016-3422-2