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Serological Analysis of Immunogenic Properties of Recombinant Meningococcus IgA1 Protease-Based Proteins.
Serological Analysis of Immunogenic Properties of Recombinant Meningococcus IgA1 Protease-Based Proteins.
- Source :
-
Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2016 Jul; Vol. 161 (3), pp. 391-4. Date of Electronic Publication: 2016 Aug 06. - Publication Year :
- 2016
-
Abstract
- Using the genome sequence of IgA1 protease of N. meningitidis of serogroup B, four recombinant proteins of different structure and molecular weight were constructed. These proteins were equal in inducing the formation of specific antibodies to IgA1 protease and had protective properties against meningococci. In the sera of immunized mice, anti-IgA1 protease antibodies were detected by whole-cell ELISA, which indicated the presence of IgA1 protease on the surface of these bacteria. We hypothesized that the protective properties of IgA1 protease-based antigens and IgA1 protease analogs could be realized not only via impairment of bacterium adhesion to the mucosa, but also via suppression of this pathogen in the organism. The presented findings seem promising for using these proteins as the basis for anti-meningococcus vaccine.
- Subjects :
- Animals
Antibodies, Bacterial immunology
Enzyme-Linked Immunosorbent Assay
Mice
Recombinant Proteins genetics
Serine Endopeptidases genetics
Serine Endopeptidases metabolism
Neisseria meningitidis immunology
Recombinant Proteins immunology
Recombinant Proteins metabolism
Serine Endopeptidases immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1573-8221
- Volume :
- 161
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Bulletin of experimental biology and medicine
- Publication Type :
- Academic Journal
- Accession number :
- 27496029
- Full Text :
- https://doi.org/10.1007/s10517-016-3422-2