Back to Search
Start Over
Chitosan silver nanocomposite (CAgNC) as an antifungal agent against Candida albicans.
- Source :
-
Medical mycology [Med Mycol] 2017 Feb 01; Vol. 55 (2), pp. 213-222. Date of Electronic Publication: 2016 Aug 04. - Publication Year :
- 2017
-
Abstract
- Due to limited numbers of antifungal drugs and emergence of drug resistance have directed to develop nonconventional therapeutic agents against fungal pathogen Candida albicans. In this study, anticandidal activity of chitosan silver nanocomposite (CAgNC) was tested against C. albicans Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of CAgNC were determined as 25 and 100 μg/ml, respectively. Electron microscopic image results confirmed the ultrastructural cell wall deformities and injuries caused by CAgNC. Propidium iodide (PI) penetration into the CAgNC treated cells could be considered as an evidence for loss of cell membrane integrity and cell death at MFC. Level of intracellular reactive oxygen species (ROS) was increased, while cell viability was decreased with the increased of CAgNC concentrations. In our protein profile results, several induced proteins were observed under CAgNC treatment, and they could be related to multidrug and stress resistant proteins such as CDR1 (55 kDa) and CaHSP70 based on the protein band size. CAgNC mediated cell wall damage, loss of cell membrane integrity, elevated ROS level, and associated oxidative stress have been identified as the main causative factors for the anticandidal activity. Overall results from our study indicated that CAgNC could affect negatively on physiological and biochemical functions of C. albicans suggesting CAgNC as a potential alternative for antifungal chemotherapy.<br /> (© The Author 2016. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Subjects :
- Antifungal Agents chemistry
Candida albicans ultrastructure
Cell Membrane drug effects
Cell Membrane physiology
Microbial Sensitivity Tests
Microbial Viability drug effects
Microscopy, Electron
Permeability drug effects
Propidium analysis
Proteome analysis
Reactive Oxygen Species analysis
Antifungal Agents pharmacology
Candida albicans drug effects
Chelating Agents pharmacology
Chitosan pharmacology
Nanocomposites chemistry
Silver pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2709
- Volume :
- 55
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Medical mycology
- Publication Type :
- Academic Journal
- Accession number :
- 27495320
- Full Text :
- https://doi.org/10.1093/mmy/myw053