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A paperless autoimmunity laboratory: myth or reality?
- Source :
-
Annales de biologie clinique [Ann Biol Clin (Paris)] 2016 Aug 01; Vol. 74 (4), pp. 477-89. - Publication Year :
- 2016
-
Abstract
- Testing for antinuclear antibodies is the most frequently prescribed analysis for the diagnosis of rheumatic diseases. Indirect immunofluorescence remains the gold standard method for their detection despite the increasing use of alternative techniques. In order to standardize the manual microscopy reading, automated acquisition and interpretation systems have emerged. This publication enables us to present our method of interpretation and characterization of antinuclear antibodies based on a cascade of analyses and to share our everyday experience of the G Sight from Menarini. The positive/negative discrimination on Hep cells 2000 is correct in 85% of the cases. In most of the false negative results, it is a question of aspecific or low titers patterns, but a few cases of SSA speckled patterns of low titers demonstrated a probability index below 8. Regarding the pattern recognition, some types and mixed patterns are not properly recognized. Concerning the probability index correlated in some studies to final titer, the weak fluorescence of certain patterns and the random presence of artifacts that distort the index don't lead us to continue it in our daily practice. In conclusion, automated reading systems facilitate the reporting of results and traceability of patterns but still require the expertise of a laboratory technologist for positive/negative discrimination and for pattern recognition.
- Subjects :
- Antibodies, Antinuclear blood
Cells, Cultured
Critical Pathways standards
Humans
Image Processing, Computer-Assisted instrumentation
Image Processing, Computer-Assisted standards
Paper
Reference Standards
Sensitivity and Specificity
Antibodies, Antinuclear analysis
Autoimmunity
Automation, Laboratory instrumentation
Automation, Laboratory methods
Automation, Laboratory standards
Laboratories standards
Laboratories trends
Subjects
Details
- Language :
- English
- ISSN :
- 1950-6112
- Volume :
- 74
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Annales de biologie clinique
- Publication Type :
- Academic Journal
- Accession number :
- 27492702
- Full Text :
- https://doi.org/10.1684/abc.2016.1164