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Highly Efficient Synthesis of Fructooligosaccharides by Extracellular Fructooligosaccharide-Producing Enzymes and Immobilized Cells of Aspergillus aculeatus M105 and Purification and Biochemical Characterization of a Fructosyltransferase from the Fungus.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2016 Aug 24; Vol. 64 (33), pp. 6425-32. Date of Electronic Publication: 2016 Aug 11. - Publication Year :
- 2016
-
Abstract
- In this work, Aspergillus aculeatus M105 was obtained to produce high extracellular fructooligosaccharide-producing enzyme activity. The maximum yields of fructooligosaccharides produced by its extracellular enzymes and immobilized cells were 67.54 and 65.47% (w/w), respectively. A fructosyltransferase (FTase), AaFT32A, was purified from M105. The optimal pH and temperature of AaFT32A were pH 5.0-6.0 and 65 °C, respectively. The Km, Vmax, and kcat values for the transfructosylating activity of AaFT32A were 2267 mM, 1347 μmol/min/mg protein, and 1550.2 s(-1), respectively, and those values for the hydrolytic activity of AaFT32A were 6.10 mM, 32.44 μmol/min/mg protein, and 37.3 s(-1), respectively. The sequence of AaFT32A deduced from the cloned gene shared 99.4% identity with a FTase from Aspergillus japonicus CB05 and a fructofuranosidase from Aspergillus niger and 96.5% identity with a FTase (Aspacl_37092) from A. aculeatus ATCC 16872. The fungal strain and its FTase may have potential applications in the prebiotics industry.
- Subjects :
- Aspergillus genetics
Chromatography, High Pressure Liquid
Cloning, Molecular
Fungal Proteins genetics
Hexosyltransferases genetics
Hydrogen-Ion Concentration
Kinetics
Mass Spectrometry
Temperature
Aspergillus enzymology
Cells, Immobilized
Fungal Proteins metabolism
Hexosyltransferases metabolism
Oligosaccharides biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 64
- Issue :
- 33
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 27492129
- Full Text :
- https://doi.org/10.1021/acs.jafc.6b02115