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Allogeneic anorectal transplantation in rats: technical considerations and preliminary results.
- Source :
-
Scientific reports [Sci Rep] 2016 Aug 04; Vol. 6, pp. 30894. Date of Electronic Publication: 2016 Aug 04. - Publication Year :
- 2016
-
Abstract
- Fecal incontinence is a challenging condition with numerous available treatment modalities. Success rates vary across these modalities, and permanent colostomy is often indicated when they fail. For these cases, a novel potential therapeutic strategy is anorectal transplantation (ATx). We performed four isogeneic (Lewis-to-Lewis) and seven allogeneic (Wistar-to-Lewis) ATx procedures. The anorectum was retrieved with a vascular pedicle containing the aorta in continuity with the inferior mesenteric artery and portal vein in continuity with the inferior mesenteric vein. In the recipient, the native anorectal segment was removed and the graft was transplanted by end-to-side aorta-aorta and porto-cava anastomoses and end-to-end colorectal anastomosis. Recipients were sacrificed at the experimental endpoint on postoperative day 30. Surviving animals resumed normal body weight gain and clinical performance within 5 days of surgery. Isografts and 42.9% of allografts achieved normal clinical evolution up to the experimental endpoint. In 57.1% of allografts, signs of immunological rejection (abdominal distention, diarrhea, and anal mucosa inflammation) were observed three weeks after transplantation. Histology revealed moderate to severe rejection in allografts and no signs of rejection in isografts. We describe a feasible model of ATx in rats, which may allow further physiological and immunologic studies.
- Subjects :
- Anastomosis, Surgical methods
Animals
Colostomy adverse effects
Male
Quality of Life
Rats
Rats, Inbred Lew
Rats, Wistar
Transplantation, Homologous
Anal Canal transplantation
Aorta transplantation
Mesenteric Artery, Inferior transplantation
Portal Vein transplantation
Plastic Surgery Procedures methods
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 27488366
- Full Text :
- https://doi.org/10.1038/srep30894