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A simultaneous diagnosis and genotyping method for global surveillance of cetacean morbillivirus.
- Source :
-
Scientific reports [Sci Rep] 2016 Aug 03; Vol. 6, pp. 30625. Date of Electronic Publication: 2016 Aug 03. - Publication Year :
- 2016
-
Abstract
- Cetacean morbillivirus (CeMV) is considered one of the most important viral pathogens in cetaceans. CeMV outbreaks of lethal disease have repeatedly been observed in Europe, the Americas, and Australia, while large herds of gregarious species were found to be the likely reservoirs and sources of CeMV infection to susceptible species in the Atlantic and Pacific Oceans. Furthermore, three new strains were detected recently in Hawaii, Brazil and Australia. To clarify the real global distribution of CeMV and possible carriers, we showed a novel technique successfully diagnosing and distinguishing different virus strains (DMV, PWMV and novel CeMVs) using FFPE samples from 1996 to 2011. This efficient method that combines qRT-PCR and high resolution melting (HRM) could be applied to the future retrospective global studies for better understanding of different prevalence and outbreak conditions among ocean basins and the mechanism of variable host response to pathogens.
- Subjects :
- Animals
Base Sequence
Brazil epidemiology
Hawaii epidemiology
Morbillivirus isolation & purification
Morbillivirus Infections epidemiology
Morbillivirus Infections veterinary
Nucleic Acid Denaturation
Spain epidemiology
Cetacea virology
Morbillivirus classification
Morbillivirus genetics
Morbillivirus Infections diagnosis
Real-Time Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 27484954
- Full Text :
- https://doi.org/10.1038/srep30625