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A data set from flash X-ray imaging of carboxysomes.
- Source :
-
Scientific data [Sci Data] 2016 Aug 01; Vol. 3, pp. 160061. Date of Electronic Publication: 2016 Aug 01. - Publication Year :
- 2016
-
Abstract
- Ultra-intense femtosecond X-ray pulses from X-ray lasers permit structural studies on single particles and biomolecules without crystals. We present a large data set on inherently heterogeneous, polyhedral carboxysome particles. Carboxysomes are cell organelles that vary in size and facilitate up to 40% of Earth's carbon fixation by cyanobacteria and certain proteobacteria. Variation in size hinders crystallization. Carboxysomes appear icosahedral in the electron microscope. A protein shell encapsulates a large number of Rubisco molecules in paracrystalline arrays inside the organelle. We used carboxysomes with a mean diameter of 115±26 nm from Halothiobacillus neapolitanus. A new aerosol sample-injector allowed us to record 70,000 low-noise diffraction patterns in 12 min. Every diffraction pattern is a unique structure measurement and high-throughput imaging allows sampling the space of structural variability. The different structures can be separated and phased directly from the diffraction data and open a way for accurate, high-throughput studies on structures and structural heterogeneity in biology and elsewhere.
Details
- Language :
- English
- ISSN :
- 2052-4463
- Volume :
- 3
- Database :
- MEDLINE
- Journal :
- Scientific data
- Publication Type :
- Academic Journal
- Accession number :
- 27479842
- Full Text :
- https://doi.org/10.1038/sdata.2016.61