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Obesity decreases the oxidant stress induced by tobacco smoke in a rat model.
- Source :
-
Toxicology mechanisms and methods [Toxicol Mech Methods] 2016 Sep; Vol. 26 (7), pp. 544-553. Date of Electronic Publication: 2016 Jul 28. - Publication Year :
- 2016
-
Abstract
- Obesity and emphysema are associated with low-grade systemic inflammation and oxidant stress. Assuming that the oxidant stress induced by emphysema would be decreased by obesity, we analyzed the oxidant/antioxidant state in a rat model combining both diseases simultaneously. Obesity was induced using sucrose, while emphysema by exposure to tobacco smoke. End-points evaluated were: body weight, abdominal fat, plasma dyslipidemia and malondialdehyde (MDA), insulin and glucose AUC, activities of Mn-superoxide dismutase (Mn-SOD), glutathione reductase (GR), glutathione transferase (GST) and glutathione peroxidase (GPx); lung MnSOD and 3-nitrotyrosine (3-NT) immunostaining, and expression of αV and β6 integrin subunits. In rats with obesity, the body weight, abdominal fat, plasma triglyceride levels, glucose AUC, insulin levels, GST activity, and αV and β6 integrin expressions were amplified. The rats with emphysema had lower values of body weight, abdominal fat, plasma insulin, triglycerides and glucose AUC but higher values of plasma MDA, GPx activity, and the lung expression of the αV and β6 integrins. The combination of obesity and emphysema compared to either condition alone led to diminished body weight, abdominal fat, plasma insulin MDA levels, GPx and GST activities, and αV and β6 integrin expressions; these parameters were all previously increased by obesity. Immunostaining for MnSOD augmented in all experimental groups, but the staining for 3-NT only increased in rats treated with tobacco alone or combined with sucrose. Results showed that obesity reduces oxidant stress and integrin expression, increasing antioxidant enzyme activities; these changes seem to partly contribute to a protective mechanism of obesity against emphysema development.
- Subjects :
- Animals
Antioxidants metabolism
Blood Glucose analysis
Emphysema chemically induced
Glucose Tolerance Test
Lipid Peroxides metabolism
Lung metabolism
Lung pathology
Male
Obesity complications
Rats, Wistar
Tobacco Smoke Pollution adverse effects
Emphysema metabolism
Lung drug effects
Obesity metabolism
Oxidative Stress drug effects
Smoke adverse effects
Nicotiana
Subjects
Details
- Language :
- English
- ISSN :
- 1537-6524
- Volume :
- 26
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Toxicology mechanisms and methods
- Publication Type :
- Academic Journal
- Accession number :
- 27470987
- Full Text :
- https://doi.org/10.1080/15376516.2016.1211208