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RCAN1 deficiency protects against Salmonella intestinal infection by modulating JNK activation.
- Source :
-
Molecular immunology [Mol Immunol] 2016 Sep; Vol. 77, pp. 26-33. Date of Electronic Publication: 2016 Jul 21. - Publication Year :
- 2016
-
Abstract
- Objective: RCAN1 (regulator of calcineurin 1) has been shown to be involved in various physiological and pathological processes. However, the biological implications of RCAN1 during gastrointestinal tract infection remain unclear. In this study, we tried to determine the role of RCAN1 in acute Salmonella infectious colitis.<br />Methods: Wild type and RCAN1-deficient mice or macrophages were used to characterize the impacts of RCAN1 on intestinal inflammation, inflammatory cytokines production, animal survival, and pathogen clearance following Salmonella challenge.<br />Results: Histologic and quantitative assessments showed increased inflammation and elevated proinflammatory cytokines production in RCAN1-deficient mice. The aberrant inflammatory response was recapitulated in primary bone marrow-derived macrophages. In addition, we reveal a novel regulatory role for RCAN1 in the proinflammatory JNK signaling both in vitro and in vivo. Further analysis showed that the increased inflammation in RCAN1-deficient mice contributed to pathogen clearance and host survival.<br />Conclusions: The present study demonstrates that RCAN1 deficiency protects against Salmonella intestinal infection by enhancing proinflammatory JNK signaling.<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Blotting, Western
Calcium-Binding Proteins
Colitis metabolism
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Intracellular Signaling Peptides and Proteins deficiency
MAP Kinase Kinase 4 metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Muscle Proteins deficiency
Salmonella Infections, Animal metabolism
Colitis immunology
Colitis microbiology
Intracellular Signaling Peptides and Proteins immunology
Muscle Proteins immunology
Salmonella Infections, Animal immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1872-9142
- Volume :
- 77
- Database :
- MEDLINE
- Journal :
- Molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 27449908
- Full Text :
- https://doi.org/10.1016/j.molimm.2016.07.009