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Novel adjuvant Alum-TLR7 significantly potentiates immune response to glycoconjugate vaccines.
- Source :
-
Scientific reports [Sci Rep] 2016 Jul 21; Vol. 6, pp. 29063. Date of Electronic Publication: 2016 Jul 21. - Publication Year :
- 2016
-
Abstract
- Although glycoconjugate vaccines are generally very efficacious, there is still a need to improve their efficacy, especially in eliciting a strong primary antibody response. We have recently described a new type of vaccine adjuvant based on a TLR7 agonist adsorbed to alum (Alum-TLR7), which is highly efficacious at enhancing immunogenicity of protein based vaccines. Since no adjuvant has been shown to potentiate the immune response to glycoconjugate vaccines in humans, we investigated if Alum-TLR7 is able to improve immunogenicity of this class of vaccines. We found that in a mouse model Alum-TLR7 greatly improved potency of a CRM197-MenC vaccine increasing anti-MenC antibody titers and serum bactericidal activity (SBA) against MenC compared to alum adjuvanted vaccine, especially with a low dose of antigen and already after a single immunization. Alum-TLR7 also drives antibody response towards Th1 isotypes. This adjuvant was also able to increase immunogenicity of all polysaccharides of a multicomponent glycoconjugate vaccine CRM197-MenACWY. Furthermore, we found that Alum-TLR7 increases anti-polysaccharide immune response even in the presence of a prior immune response against the carrier protein. Finally, we demonstrate that Alum-TLR7 adjuvant effect requires a functional TLR7. Taken together, our data support the use of Alum-TLR7 as adjuvant for glycoconjugate vaccines.
- Subjects :
- Adjuvants, Immunologic chemistry
Aluminum Hydroxide administration & dosage
Aluminum Hydroxide chemistry
Animals
Bacterial Proteins chemistry
Bacterial Proteins immunology
Female
Glycoconjugates chemistry
Humans
Immunogenicity, Vaccine
Immunoglobulin G biosynthesis
Meningitis, Meningococcal immunology
Meningitis, Meningococcal microbiology
Meningococcal Vaccines biosynthesis
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Neisseria meningitidis drug effects
Neisseria meningitidis immunology
Toll-Like Receptor 7 chemistry
Vaccination
Vaccines, Conjugate administration & dosage
Vaccines, Conjugate biosynthesis
Adjuvants, Immunologic administration & dosage
Antibodies, Bacterial biosynthesis
Glycoconjugates administration & dosage
Meningitis, Meningococcal prevention & control
Meningococcal Vaccines administration & dosage
Toll-Like Receptor 7 administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 27439378
- Full Text :
- https://doi.org/10.1038/srep29063