Back to Search Start Over

Exploration of steroidogenesis-related genes in testes, ovaries, adrenals, liver and adipose tissue in pigs.

Authors :
Robic A
Feve K
Louveau I
Riquet J
Prunier A
Source :
Animal science journal = Nihon chikusan Gakkaiho [Anim Sci J] 2016 Aug; Vol. 87 (8), pp. 1041-7. Date of Electronic Publication: 2015 Dec 21.
Publication Year :
2016

Abstract

To explore the metabolism of steroids in the pig species, a qualitative PCR analysis was performed for the main transcript of 27 genes involved in steroid metabolism. We compared samples of testes, adipose tissue and liver from immature and peripubertal males, adrenal cortex from peripubertal males, ovaries from cyclic females and adipose tissue from peripubertal females. Some genes were shown to have a tissue-specific expression. Two of them were expressed only in testes, ovaries and adrenals: CYP11A1 and CYP11B. The CYP21 and HSD17B3 genes, were expressed respectively only in adrenals and only in testes. Very few differences were observed between transcriptional patterns of peripubertal testes and adrenal glands as well as between male and female fat tissues. However, the expression of genes involved in the sulfonation of steroids was higher in testes than in adrenals from males. Main differences between ovaries and testes were observed for HSD17B1/2/3, AKR1C-pig6 and sulfotransferase genes (SULT2A1/SULT2B1). The present study shows that the SRD5A2 and CYP21 genes were not involved in the testicular biosynthesis of androstenone. It also shows that porcine adrenal glands produce essentially corticosteroids and that fat tissue is unable to produce de novo steroids.<br /> (© 2015 Japanese Society of Animal Science.)

Details

Language :
English
ISSN :
1740-0929
Volume :
87
Issue :
8
Database :
MEDLINE
Journal :
Animal science journal = Nihon chikusan Gakkaiho
Publication Type :
Academic Journal
Accession number :
27436769
Full Text :
https://doi.org/10.1111/asj.12532