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Exploration of steroidogenesis-related genes in testes, ovaries, adrenals, liver and adipose tissue in pigs.
- Source :
-
Animal science journal = Nihon chikusan Gakkaiho [Anim Sci J] 2016 Aug; Vol. 87 (8), pp. 1041-7. Date of Electronic Publication: 2015 Dec 21. - Publication Year :
- 2016
-
Abstract
- To explore the metabolism of steroids in the pig species, a qualitative PCR analysis was performed for the main transcript of 27 genes involved in steroid metabolism. We compared samples of testes, adipose tissue and liver from immature and peripubertal males, adrenal cortex from peripubertal males, ovaries from cyclic females and adipose tissue from peripubertal females. Some genes were shown to have a tissue-specific expression. Two of them were expressed only in testes, ovaries and adrenals: CYP11A1 and CYP11B. The CYP21 and HSD17B3 genes, were expressed respectively only in adrenals and only in testes. Very few differences were observed between transcriptional patterns of peripubertal testes and adrenal glands as well as between male and female fat tissues. However, the expression of genes involved in the sulfonation of steroids was higher in testes than in adrenals from males. Main differences between ovaries and testes were observed for HSD17B1/2/3, AKR1C-pig6 and sulfotransferase genes (SULT2A1/SULT2B1). The present study shows that the SRD5A2 and CYP21 genes were not involved in the testicular biosynthesis of androstenone. It also shows that porcine adrenal glands produce essentially corticosteroids and that fat tissue is unable to produce de novo steroids.<br /> (© 2015 Japanese Society of Animal Science.)
- Subjects :
- 17-Hydroxysteroid Dehydrogenases genetics
17-Hydroxysteroid Dehydrogenases metabolism
20-Hydroxysteroid Dehydrogenases genetics
20-Hydroxysteroid Dehydrogenases metabolism
Animals
Cholesterol Side-Chain Cleavage Enzyme genetics
Cholesterol Side-Chain Cleavage Enzyme metabolism
Cytochrome P450 Family 21 genetics
Cytochrome P450 Family 21 metabolism
Female
Male
Organ Specificity genetics
Steroid 11-beta-Hydroxylase genetics
Steroid 11-beta-Hydroxylase metabolism
Sulfotransferases genetics
Sulfotransferases metabolism
Adipose Tissue metabolism
Adrenal Glands metabolism
Gene Expression
Gene Expression Regulation, Enzymologic
Liver metabolism
Ovary metabolism
Steroids biosynthesis
Swine genetics
Swine metabolism
Testis metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1740-0929
- Volume :
- 87
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Animal science journal = Nihon chikusan Gakkaiho
- Publication Type :
- Academic Journal
- Accession number :
- 27436769
- Full Text :
- https://doi.org/10.1111/asj.12532