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Molecular cloning and functional characterization of porcine E74-like factor 4 (ELF4).
- Source :
-
Developmental and comparative immunology [Dev Comp Immunol] 2016 Dec; Vol. 65, pp. 149-158. Date of Electronic Publication: 2016 Jul 15. - Publication Year :
- 2016
-
Abstract
- E74-like factor 4 (ELF4) is a novel transcription factor that initiates transcription of type I interferon (IFN) genes to control diverse pathogens. Here, porcine ELF4 (poELF4) was cloned and its role in type I IFN signaling was investigated in different porcine cell lines. Full-length cDNA of poELF4 encodes 663 amino acid residues and ectopic expression of poELF4 significantly induced IFN-β production. Interestingly, difference from the human ELF4 (huELF4), poELF4 mutants lacking the serine/threonine rich domain, which has been demonstrated to be responsible for the phosphorylation of huELF4, were still capable of activating IFN-β promoter. Using pseudorabies virus (PRV) and porcine reproductive and respiratory syndrome virus (PRRSV) as the models of DNA virus and RNA virus, respectively, we found that the replication of both PRV and PRRSV was reduced with poELF4 overexpression and enhanced with poELF4 knockdown. Taken together, these results suggested that poELF4 is an important antiviral host restriction factor.<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Cell Line
Cloning, Molecular
DNA-Binding Proteins genetics
Host-Pathogen Interactions
Immunity, Innate
Mutation genetics
Promoter Regions, Genetic genetics
Protein Domains genetics
Signal Transduction
Transcription Factors genetics
Herpesvirus 1, Suid immunology
Interferon Type I metabolism
Pseudorabies immunology
Swine immunology
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0089
- Volume :
- 65
- Database :
- MEDLINE
- Journal :
- Developmental and comparative immunology
- Publication Type :
- Academic Journal
- Accession number :
- 27426928
- Full Text :
- https://doi.org/10.1016/j.dci.2016.07.005